Normal Mutant Paper chromatography +. Paper chromatography Electrophoresis at pH = 6.5 Electrophoresis at pH = 6.5

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A normal polypeptide and a mutant of the polypeptide were hydrolyzed by an endopeptidase under the same conditions. The normal and mutant
poly peptide differ by one amino acid. The fingerprints of the peptides obtained from the two polypeptides are shown below. What kind of amino acid substitution occurred as a result of the mutation? (That is, is the substituted amino acid more or less polar than the original amino acid? Is its pI lower or higher?)

Normal
Mutant
Paper chromatography
+.
Paper chromatography
Electrophoresis at pH = 6.5
Electrophoresis at pH = 6.5
Transcribed Image Text:Normal Mutant Paper chromatography +. Paper chromatography Electrophoresis at pH = 6.5 Electrophoresis at pH = 6.5
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