Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18) bonded phase. The mobile phase was 65 vol% methanol in water. How would the retention times of these aromatic compounds be affected if 90 vol% methanol was used instead? The retention times will be longer in 90 vol% methanol. The retention times will double. The retention times will not change. The retention times will be shorter in 90 vol% methanol.

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Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18)
bonded phase. The mobile phase was 65 vol% methanol in water. How would the
retention times of these aromatic compounds be affected if 90 vol% methanol was
used instead?
The retention times will be longer in 90 vol% methanol.
The retention times will double.
The retention times will not change.
The retention times will be shorter in 90 vol% methanol.
Transcribed Image Text:Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18) bonded phase. The mobile phase was 65 vol% methanol in water. How would the retention times of these aromatic compounds be affected if 90 vol% methanol was used instead? The retention times will be longer in 90 vol% methanol. The retention times will double. The retention times will not change. The retention times will be shorter in 90 vol% methanol.
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