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Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Solve full accurate dont use guideline answer ok
Solve full accurate with diagrams okk.

Transcribed Image Text:Mitchell presented theoretical calculations to show how a reversible ATPase system located in a membrane
could separate charges on the outside and inside of the membrane. Based on his calculations, he came
up with the diagram in Figure 4.
Questions
1. Based on the diagram, is the overall charge
outside the membrane positive or negative?
2. Based on the diagram, is the overall charge
inside the membrane positive or negative?
3. Which side of the membrane has a lower pH?
4. Which side of the membrane is more acidic?
Inside (L) Membrane
ОН'
Outside (R)
ADP-0
POH
H*
ADP-O-P*
Figure 4. The original caption for this figure in Mitchell (1961)
reads: "Fig. 1. Anisotropic reversible ATPase' system located in
an ion-impermeable membrane between aqueous phases L and
R." Credit: Reprinted by permission from Macmillan Publishers
Ltd: Nature 1961.
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