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Mitchell presented theoretical calculations to show how a reversible ATPase system located in a membrane could separate charges on the outside and inside of the membrane. Based on his calculations, he came up with the diagram in Figure 4. Questions 1. Based on the diagram, is the overall charge outside the membrane positive or negative? 2. Based on the diagram, is the overall charge inside the membrane positive or negative? 3. Which side of the membrane has a lower pH? 4. Which side of the membrane is more acidic? Inside (L) Membrane ОН' Outside (R) ADP-0 POH →H* ADP-O-P+ Figure 4. The original caption for this figure in Mitchell (1961) reads: "Fig. 1. Anisotropic reversible ATPase' system located in an ion-impermeable membrane between aqueous phases L and R." Credit: Reprinted by permission from Macmillan Publishers Ltd: Nature 1961.

Mitchell presented theoretical calculations to show how a reversible ATPase system located in a membrane could separate charges on the outside and inside of the membrane. Based on his calculations, he came up with the diagram in Figure 4. Questions 1. Based on the diagram, is the overall charge outside the membrane positive or negative? 2. Based on the diagram, is the overall charge inside the membrane positive or negative? 3. Which side of the membrane has a lower pH? 4. Which side of the membrane is more acidic? Inside (L) Membrane ОН' Outside (R) ADP-0 POH →H* ADP-O-P+ Figure 4. The original caption for this figure in Mitchell (1961) reads: "Fig. 1. Anisotropic reversible ATPase' system located in an ion-impermeable membrane between aqueous phases L and R." Credit: Reprinted by permission from Macmillan Publishers Ltd: Nature 1961.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Mitchell presented theoretical calculations to show how a reversible ATPase system located in a membrane could separate charges on the outside and inside of the membrane. Based on his calculations, he came up with the diagram in Figure 4. Questions 1. Based on the diagram, is the overall charge outside the membrane positive or negative? 2. Based on the diagram, is the overall charge inside the membrane positive or negative? 3. Which side of the membrane has a lower pH? 4. Which side of the membrane is more acidic? Inside (L) Membrane ОН' Outside (R) ADP-0 POH H* ADP-O-P* Figure 4. The original caption for this figure in Mitchell (1961) reads: "Fig. 1. Anisotropic reversible ATPase' system located in an ion-impermeable membrane between aqueous phases L and R." Credit: Reprinted by permission from Macmillan Publishers Ltd: Nature 1961.
Transcribed Image Text:Mitchell presented theoretical calculations to show how a reversible ATPase system located in a membrane could separate charges on the outside and inside of the membrane. Based on his calculations, he came up with the diagram in Figure 4. Questions 1. Based on the diagram, is the overall charge outside the membrane positive or negative? 2. Based on the diagram, is the overall charge inside the membrane positive or negative? 3. Which side of the membrane has a lower pH? 4. Which side of the membrane is more acidic? Inside (L) Membrane ОН' Outside (R) ADP-0 POH H* ADP-O-P* Figure 4. The original caption for this figure in Mitchell (1961) reads: "Fig. 1. Anisotropic reversible ATPase' system located in an ion-impermeable membrane between aqueous phases L and R." Credit: Reprinted by permission from Macmillan Publishers Ltd: Nature 1961.
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