Methylcyclohexane and methylcyclohexene were separated by gas chromatography in a capillary column, which showed retention times of 10.0 and 10.9 minutes, respectively. The width at base were found to be 0.76 and 0.82
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- A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μmA student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc, was found to be 209.6 s with a baseline peak width, w., of 14.4 s. The retention time for aspartame, ta, was 260.0 s with a baseline peak width, wa, of 21.2 s. The retention time for the unretained solvent methanol was 49.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = um %3D Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2. R2N =An unknown compound was analyzed by GC with octane (C3H18) and decane (C10H22). The adjusted retention times were 14.6 min for octane, and 31.6 min for decane. Calculate the retention index for the unknown if its adjusted retention time was 21.2 min.
- A solute with a retention time of 407 seconds has a width at the base of 13.0 seconds on a column 12.2 meters long. Find the number of plates and plate height. The half-width of the same peak is 7.6 seconds. Find the plate height.A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase1. Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385 s, respectively, and half widths (W₁/2) of 9.42 and 10.0 s. An unretained butane peak occurs at 10.0 s. Calculate the separation factor and the resolution.
- In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesAn unretained solute is eluted from a GC column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecan (C11H24) is eluted in 17.22 min. What is the retention time (report to 2 decimal places, in minutes) of a compound whose retention index is 1050?Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18) bonded phase. The mobile phase was 65 vol% methanol in water. How would the retention times of these aromatic compounds be affected if 90 vol% methanol was used instead? The retention times will be longer in 90 vol% methanol. The retention times will double. The retention times will not change. The retention times will be shorter in 90 vol% methanol.
- A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure. Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places) What other dye/s are present in dye C and what is the dye with least number of pigments?Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.Benzene and toluene, when analysed isothermally on a 25 m × 0.25 mm i.d. capillary GC column, were found to have retention times of 8.56 and 8.67 min, respectively. An unretained solvent passed through the column in 4.10 min in the same analysis. The peak widths at half height for benzene and toluene were 4.2 and 4.3 seconds, respectively. Calculate: a) The capacity factor for each of these compounds b) The selectivity factor for the separation c) The average number of plates for the column d) The resolution between benzene and toluene. Are they completely separated under these conditions? e) What is the plate height (H) for this column? What can you say about the efficiency of this column? Explain f) The dimensions of a column with the same phase ratio required to obtain a resolution of 1.5 under otherwise identical conditions