Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN: 9781133893943
Author: ESTRIDGE
Publisher: Cengage
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Medically Significant Bacteria: Causative Agent and Disease Profile

  

ITEM

IMPORTANCE OF BACTERIA

PROFILE

 

BACTERIAL PROFILE

 

I

MICROORGANISM/CAUSATIVE AGENT

 

A

GRAM REACTION

 

B

OXYGEN REQUIREMENT

 

C

SIZE

 

D

SHAPE

 

E

HABITAT

 

F

DISCOVERY

 

G

MICROSCOPIC IMAGE

 

 

 

 

II

DISEASE PROFILE

 

A

DISEASE/S

 

B

SYMPTOMS OF THE DISEASE

 

C

INCUBATION PERIOD

 

D

MODE OF TRANSMISSION

 

E

DIAGNOSIS

 

F

TREATMENT

 

G

PREVENTION

 

H

NO OF DAYS BEING SYMPTOMATIC

 

 

 

 

I

IMAGE OF INFECTED PATIENT

 

 

GENERAL INSTRUCTIONS:

Using the template provided above, fill out the information for a bacteria. YOU CAN CHOOSE ANY BACTERIA from 1-60 BELOW:

  1. List of medically significant bacteria

Aerobic Gram (+) Cocci

  1. S. epidermidis
  2. S. saprophyticus
  3. Streptococcus pyogenes
  4. Streptococcus agalactiae
  5. Enterococcus(E. faecalis, E. facium)

 

Gram Positive Bacilli

  1. Corynebacterium diptheriae(“Kleb-Loefflers” Bacillus)
  2. Corynebacterium jeikeium
  3. Listeria monocytogenes
  4. Erysipelothrix rhusiopathiae
  5. Arcanobacterium haemolyticum 13.Gardnerella vaginalis
  6. Nocardia spp.
  7. Bacillus anthracis
  8. Bacillus cereus
  9. Clostridium perfringens
  10. Clostridium botulinum

 

Gram Negative Diplococci

  1. Neisseria gonorrhoeae
  2. Neisseria meningitidis
  3. Moraxella catarrhalis

 

Gram Negative Bacilli or coccobacilli (Fastidious, MacConkey Negative)

  1. H. influenzae(Pfeiffer’s bacillus)
  2. H. aegyptius(Koch-Weeks bacillus) / H. influenzae bio. aegyptius
  3. H. ducreyi-Chancroid (soft chancre)
  4. Brucella spp.-Brucellosis or Undulant fever
  5. Francisella tularensis-Tularemia (ulcero-, oro-, glandular ; pneumonic)
  6. Legionella pneumophila-Legionnaire’s disease (febrile disease with pneumonia)
  7. Bordetella pertussis -Pertussis
  8. Brucella melitensis
  9. Haemophilus ducreyi

 

Gram Negative Bacilli (Oxidative, Mac Conkey positive)

 

  1. Pseudomonas aeruginosa
  2. Acinetobacter spp.
  3. P. fluorescens
  4. B. pseudomallei
  5. B. cepacian

 

Gram – Bacilli (Fermentative, Mackonkey Positive. Oxidase negative) Enterobacteriaceae, Vibrio, Aeromonas, Plesiomonas

  1. P. shigelloides
  2. Klebsiella & Shigella
  3. Escherichia coli  Uropathogenic E. coli

Enterotoxigenic E. coli (ETEC)

Enteroinvasive E. coli (EIEC)

Enterophatogenic E. coli (EPEC)

Enterohemorrhagic E. coli (EHEC)

Enteroadherent E. coli (EAEC)

  1. Klebsiella(Friedlander)
  2. pneumoniaesubs. pneumoniae

                             subs. oxytoca

  1. Enterobacter
  2. aerogenes
  3. cloacae
  4. Serratia  marcescens

 

  1. Citrobacter spp.
  2. Procteus spp.

Proteus vulgaris

Proteus mirabilis

  1. Providencia

Prov. stuartii

Prov. retgerri

  1. Morganella morganii
  2. Salmonella

Salmonella Typhi

  1. Paratyphi 
  2. Chloraesuis

Salmonella enterica subsp. enterica

  1. Shigella
  2. dysenteriae
  3. flexneri
  4. boydii
  5. sonnei
  6. Yersinia pestis
  7. Yersinia enterocolitica

 

Gram Negative Bacilli Fermenter

  1. Vibrio spp.

Vibrio cholerae

Vibrio parahaemolyticus

Vibrio vulnificus

Vibrio alginolyticus

Vibrio mimicus

 

  1. Aeromonas hydrophila
  2. Plesiomonas shigelloides

Spirochetes

  1. Leptospira interrogans
  2. Borrelia spp.

Borrelia recurrentis

Borrelia burgdorferi sensu lato

  1. Treponema pallidumsubs pallidum

Others

  1. Chlamydia

 Chlamydia trachomatis

Chlamydophila pneumoniae

Chlamydophila psittaci

  1. Rickettsiaspp
  2. akari    
  3. conorii
  4. rickettsia
  5. prowazekii
  6. typh
  7. Orientia tsutsugamushi

Mycobacteria

  1. M. tuberculosis
  2. M. bovisand M. bovis BCG

Clostridium perfringens (Epsilon Toxin) 

 

REFER TO THE EXAMPLE BELOW ON HOW TO DO IT. PLEASE INCLUDE THE REFERENCES AT THE LAST PAGE

 

Medically Significant Bacteria: Causative Agent and Disease Profile for S. aureus

 

A.     Template and Example

 

ITEM

MSM

PROFILE

 

MICROBIAL PROFILE

 

I

MICROORGANISM/CAUSATIVE AGENT

Staphylococcus aureus

A

GRAM REACTION

(+)

B

OXYGEN REQUIREMENT

Facultative Aerobes

C

SIZE

1.5 µm

D

SHAPE

Cocci in clusters

E

HABITAT

Normal flora of skin/anterior nares/pharynx

F

DISCOVERY

 

G

MICROSCOPIC IMAGE

 

II

DISEASE PROFILE

Scalded skin syndrome

A

DISEASE/S

                       Skin and Wound Infections      

Scalded Skin Syndrome

Toxic Shock Syndrome

Food Poisoning Pneumonia

B

SYMPTOMS OF THE DISEASE

A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn

C

INCUBATION PERIOD

2 and 4 hours (range 30 minutes to 8 hours)

D

MODE OF TRANSMISSION

Contaminated object, droplets sneezing or coughing, 

E

DIAGNOSIS

Biochemical Test Staphylocoagulase (Coagulase) +

 

 

 

Staphylokinase (Fibrinolysin) +

Protease, Hyaluronidase, Lipase +

Gram +, Catalase +, Oxidase -, Cocci

Cultural morphology Sheeps Blood Agar appearance: β-hemolytic-Medium to large,Pigmented yellow ɣ-hemolytic -Small to medium,Gray-white Mannitol Salt Agar (MSA) appearance

Growth with fermentation       (yellow halos)

 

testing colonies for agglutination with latex particles

coated with immunoglobulin G and fibrinogen which

bind protein A and the clumping factor,

F

TREATMENT

Antibiotics commonly prescribed to treat staph infections include certain cephalosporins such as cefazolin; nafcillin or oxacillin; vancomycin; daptomycin (Cubicin); telavancin (Vibativ); or linezolid (Zyvox).

G

PREVENTION

Keep your hands clean by washing them thoroughly with soap and water. Or use an alcohol-based hand sanitizer. Keep cuts and scrapes clean and covered

with bandages until they heal. Avoid contact with other people's wounds or bandages

 

H

NO OF DAYS BEING SYMPTOMATIC

4-10 days

 

 

 

I

IMAGE OF INFECTED PATIENT

 

 

 

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