Lambda (A) ref merical apertur which the light a line from each for each lens ar of each objectiv
Lambda (A) ref merical apertur which the light a line from each for each lens ar of each objectiv
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Question
Explain the first row of number and ignore the rest
Transcribed Image Text:Tuctionatiun
33
Not
D times the sine of 1/2 of the angle formed by projecting
%3D
which the light is traveling (air
a line from each edge of the objective lens to the focal point on the slide. The NA is constant
for each lens and is stamped on the side of the objective by the manufacturer. Find the NA
of each objective lens on your microscope and record the value below.
10X:
20X:
40X:
4X:
Assuming that the average wavelength of visible light is 546 nm, calculate the resolution in
nm for each of the objective lenses.
4X:
10X:
20X:
40X:
The smaller the value of R, the better the resolution of that lens. Which color of visible
nght (ranging from 400 nm blue light to 700 nm red light) would give the best resolution:
Show
your calculations.
Lambda (À) refers to the of in and NA is the nu-
merical of the lens. NA is as the index of the through
Expert Solution
Concept and Principle:
For a microscope, the numerical aperture is the number that defines the resolving power of the microscope. Resolving power is the ability to distinguish two objects from each other. Higher the resolving power better the quality.
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