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- Which of the following statements regarding microbial control agents is FALSE? Question options: strongly acidic or alkaline disinfectants are typically more effective than neutral pH agents the presence of extracellular fats typically decreases the effectiveness of control agents a warmed disinfectant is typically more effective than the same disinfectant at room temperature substances secreted by members of biofilms increase protection against microbial control agents all of these statements are TRUE bacterial endospores are more resistant to antimicrobial treatment than vegetative bacterial cellsA class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LA. Bacterial Growth Curve Categorization. Drag and drop the microbial culture plates on the column that best represents the bacterial colony growth phase. -Number of Bacterial Cells (Log) ➡➡➡ Lag Exponential Phase Phase Stationary Phase Time (h) 24 Death Phase 48
- There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D CAntibiotics should be reduced or eliminated from culture media during cell subculturing. Make it more detailed.
- A lab technician is working with a bacterium in pure culture (in 5 ml of liquid media in a test tube). The bacterium is a mesophile that can infect humans. Which of the following is NOT true (with regards to temperature conditions for this bacterial culture)? Lowering the temperature to -10 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 37 deg C will likely facilitate rapid growth of the bacteria. Raising the temperature to 90 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 4 deg C will likely slow or halt growth of the bacteria.A patient presents with sepsis from an E.coli bacteremia (bacteria growing in bloodstream). E. coli is a Gram-negative Enterobacteriaceae. You perform antibiotic sensitivity tests using the Kirby Bauer disc diffusion technique. The diameter of the zone of Inhibition for Ampicillin is for the E. coli is 17 mm. Is the E.coli strain sensitive, intermediate or resistant to ampicillin at therapeutic levels? Zone of Inhibition Diameter Interpretive Chart in mm for Ampicillin Resistant Intermediate Sensitive Enterobacteriaceae 17 >29 Staphylococcus <28Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.
- In the laboratory, autoclaving is required to prepare media for the growth of microorganism. What type of method of control does this involve? Dry Heat Moist Heat Chemical Radiation All of the optionsMy professor instructed me to make presentation on this topic "Periodontal microbes interaction with FISH". I have to make 4 power point slides regarding this topic. Can you give some information about the topic which i can use in the slides? Also provide some necessary relevant diagram or image regarding the topic. Please answer at your own easy words. If you do so i will rate you positive, Thank you.The following cultures are the same bacteria grown at different temperatures. Based on these results, which culture represents the ideal growing temperature? S.marcescens Culture A (left) Culture B (right) RT 3/3/16 37% marusurs 3/4/2016 com.co
