An enzyme is needed to be stored in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory:Buffer SystemWeak Acid ComponentKaConjugate Base SaltPAHBenzoic acid, C6H5COOH6.25 x 10-5NaC6H5COOSADBoric acid, H3BO35.81 x 10-7NaH2BO3DOHHydrogen cyanide, HCN6.20 X 10-10NaCN In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? (show calculations and briefly explain)Write the equilibrium reaction involved in the buffer system.Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.

The pH of a buffer solution can be determined by Henderson's equation given below. pH=pKa +…

Answered: In what buffer system (PAH, SAD, or…

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter11: Chemical Kinetics: Rates Of Reactions

Section: Chapter Questions

Problem 83QRT

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An enzyme is needed to be stored in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory:

Buffer System	Weak Acid Component	K_a	Conjugate Base Salt
PAH	Benzoic acid, C₆H₅COOH	6.25 x 10^-5	NaC₆H₅COO
SAD	Boric acid, H₃BO₃	5.81 x 10^-7	NaH₂BO₃
DOH	Hydrogen cyanide, HCN	6.20 X 10^-10	NaCN

In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? (show calculations and briefly explain)
Write the equilibrium reaction involved in the buffer system.
Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.

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