Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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In the equation describing Tm of DNA: Tm = 81.6 +0.41(GC %) + 16.6 log [C], the
term 81.6 °C represents a constant i.e. the temperature necessary to melt dsDNA at
any salt concentration and GC content. This temperature is
required for:
a. Breaking hydrogen bonds between bases.
b. Breaking hydrogen bonds between GC pairs.
c. Stabilizing conformation of single-stranded DNA.
d. Breaking stacking interaction between bases results in the unwinding
double-helix structure of DNA existing in B crystallographic conformation
followed by breaking hydrogen bonds between bases.
e. Breaking stacking interaction between bases, resulting in the unwinding
double-helix structure of DNA existing in A crystallographic
conformation.
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Transcribed Image Text:In the equation describing Tm of DNA: Tm = 81.6 +0.41(GC %) + 16.6 log [C], the term 81.6 °C represents a constant i.e. the temperature necessary to melt dsDNA at any salt concentration and GC content. This temperature is required for: a. Breaking hydrogen bonds between bases. b. Breaking hydrogen bonds between GC pairs. c. Stabilizing conformation of single-stranded DNA. d. Breaking stacking interaction between bases results in the unwinding double-helix structure of DNA existing in B crystallographic conformation followed by breaking hydrogen bonds between bases. e. Breaking stacking interaction between bases, resulting in the unwinding double-helix structure of DNA existing in A crystallographic conformation.
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