if you are running quantitative test where serum is diluted 6 times and you observed agglutination till the last dilution what does that mean? Does there a specific number for antibody titre?

What is the minimum number of reactive antigen-positive and non-reactive antigen-negative panel cells that must be present in order to identify a red cell antibody with statistical confidence?   Please select the single best answer   Two   Three   Five   Ten

What is a secondary antibody in an ELISA test?

After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound to 1st Antibody bound to 2nd Antibody?

What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?

In some cases, the design of the ELISA method is slightly different. The wells arecoated with anti-SARS-CoV-2 spike protein antibodies, serum is added, and thenanother set of anti-SARS-CoV-2 spike protein antibodies with enzyme conjugated tothem is added. What is being assayed for in this procedure and what advantage mightit have in clinical terms

Give the perfect answer  with step by step Answer.

You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).

How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?

Please tell me the names of the antibodies you would need to buy if your antigen is GAPDH.  You can make up the species of the animals.