if you are running quantitative test where serum is diluted 6 times and you observed agglutination till the last dilution what does that mean? Does there a specific number for antibody titre?

Q: What is the purpose of an agglutination reaction test?

A: Antigen are the foreign substances to the body in the form of proteins, carbohydrates which may…

Q: d Sep 2 Typing Vou are solving for an unknown blood type. Drops of blood have been mixed with…

A: The method of identifying the blood type in an individual is called blood typing. It is important to…

Q: Why do you coat the elisa plate with albumin?

A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…

Q: direct fluorescent antibody, ouchterlony diffusion, ELISA) which do you think would work best for…

A: Blood grouping Blood grouping is the classification of the blood based on the type of antigen/s that…

Q: In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s…

A: In direct method of antigen detection, the primary antibody is directly attached to a label. It…

Q: If the protein produced full length antibody what would be the next step after centrifugation?

A: Antibodies are protein related to immune system know as Immunoglobulin. Each antibody is made up of…

Q: In the first panel of part (a), what is the purpose of the antibodies attached to the well?

A: Enzyme-linked immunosorbent assay (ELISA) is a disease detection method (AIDS) and it works on the…

Q: What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays?…

A: Introduction  The main property of our immune cells is that of specificity. They are highly specific…

Q: What is the significance of a rise in titer of specific antibodies in serum samples taken at…

A: Antibodies or immunoglobulins are the Y-shaped molecules that are responsible for binding to the…

Q: What is the role of horseradish peroxidase conjugate in the ELISA experiment?

A: Horseradish peroxidase  The enzyme horseradish peroxidase (HRP), located in horseradish's roots, is…

Q: What are the different solid phases that antibody or antigen can bind to, in ELISA?

A: Quantitative immunological techniques that use a solid phase can be defined as solid-phase…

Q: How would you determine the optimal antigen: antibody ratio by means ofthe ring test?

A: Serology is a branch of medicine that deals with utilization of the blood samples in the laboratory…

Q: The secondary antibody used in the laboratory for western blotting is conjugated to which enzyme?…

A: Secondary antibody is complementary to the primary antibody that is specific for a particular…

Q: You performed an ELISA and upon looking at your results, you realized you torgot to coat the wells…

A: A key feature of the plate based ELISA is that capture protein that is antibody or antigen can be…

Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…

A:

Q: Why do you think defibrnated blood is commonly used as the representative of blood sample in most…

A: The blood possesses an insoluble protein called fibrin, which is formed from fibrinogen produced by…

Q: If the highest dilution with a color change in an ELISA test is 1:64, the antibody titer is 640.…

A: ELISA stands for enzyme linked immune-sorbent assay. It is a technique used for the identification…

Q: In the complement fixation test (CFT) , Once sRBC has been pipetted into the wells below, please…

A: The question is asking about the observations we would make in a complement fixation test (CFT) when…

Q: Can you please help me with this chart and explain what serum antibodies are?

A: ABO blood types used to identify the presence of antigens. There could be the presence of one, both…

Q: There are five major classes of antibodies. Complete the three below.

A: An antibody, also known as an immunoglobulin, is a large, Y-shaped protein used by the immune system…

Q: The technician decided to antigen type the patient to confirm predictions from the antibody panel.…

A: The objective of the question is to determine which statement is best supported by the given antigen…

Q: Predict the outcome of an error in ELISA protocol consisting of adding the antibodies in the wrong…

A: ELISA stands for enzyme linked immunosorbant assay.This techniques is basically used for the…

Q: If the test samples are positive for the antigen in the ELISA we are using, what color will you see?…

A: ELISA:                     ELISA ~ Enzyme-Linked Immunosorbent Assay          In analytical…

Q: The conjugated -antibody can be either primary antibody or the secondary antibody True False

A: Primary antibody binds to antigen while secondary antibodies bind to primary antibody Fc region. Fc…

Q: An ELISA was performed on the sera of three patients to detect antibody against varicella (the…

A: A sample of the patient's blood is obtained and then confronted with known antigens to see if…

Q: If, during blood typing, you observe positive reaction with anti- B serum, that person's blood has…

A: The determination of the blood group is called ABO typing. Whenever a person needs a transfusion of…

Q: An ELISA test is useful for detecting the presence of antigens but not antibodies in a sample.…

A: The enzyme-linked immunosorbent assay (ELISA) test is used to diagnose HIV which causes AIDS. It is…

Q: Difference between Antibody ELISA and Antigen ELISA

A: Introduction Enzyme-linked immunoassay (ELISA) is an acronym for enzyme-linked immunoassay.…

Q: You performed an elisa test in the lab to detect antibody against HIV in the patients serum. The…

A:

Q: Describe the method, time frame, and rationale for the tuberculinskin test. What component of the…

A: The tuberculin skin test is also known as Mendel–Mantoux test or Mantoux test. It is a tool used for…

Q: The sandwich type of ELISA detects patient antibodies to an antigen.

A: Antigen and antibody reactions are specific. The epitope of the antigen binds to the paratope of the…

Q: Name two types of immunodiffusion tests and describe how they operate

A: Immunodiffusion is a test which involves the use of soft agar or agarose to allow diffusion of…

Q: What is the principle of Immunochromatographic system, and its uses? Long anwser

A: Immunochromatography is the combination of immunoassay ( which is defined as biochemical tests that…

Q: Patient Testing Solution (Mr. Smith) Possible Genotypes for blood type Reaction Phenotype (Blood…

A: Blood group of an individual can be evaluated by presence/absence of certain proteins on surface of…

Q: A researcher is following a protocol for immunohistochemical staining of meniscus using a goat…

A: Introduction : Using antibodies that recognise and attach to the antigens, the laboratory technique…

Q: Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid…

A: The objective of the question is to understand if rapid antibody testing is a form of ELISA…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.