If the lacl gene is mutated so that the repressor protein no longer binds to lactose, what will be the effect on the expression of B-galactosidase in lactose's presence and absence? Explain.

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: What would happen if the operator sequence of the lac operon contained a mutation that prevented the…

A: The Structure Of lac operon --Genes present in the lac operon specify proteins which help in the…

Q: Diagram the lactose operon in E. coli with the properorder of the elements lacI, lacO, lacY, and…

A: An operon is a group of genes that are expressed together under a common promoter and terminator.…

Q: Explain why catabolite repression is used in regulating the lac operon and describe how it…

A: An Operon is a unit of structure and function that consists of the clusters of regulatory genes…

Q: Describe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite…

A: Operon is a functional unit of DNA that consists of cluster of genes under control of promoter.…

Q: The genes that encode the enzymes for arginine biosynthesis are located at several positions around…

A: A gene is a stretch of nucleotides present on a chromosome. It is the region that encodes the…

Q: How would a mutation which prevented Gal3 from binding to Gal80 affect gene expression from the GAL…

A: Galactose metabolizing genes express themselves in the presence of galactose. Galactose is…

Q: The trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino…

A: When tryptophan levels are low tryptophan operon  is induced to transcribe structural genes so that…

Q: Explain why (a) inactivation of the O2 or O3 sequence of the lac operon causes only a twofold loss…

A: An operon is defined as a group of genes that have a common promoter and regulator and also…

Q: A mutation occurred on the lacI (repressor) gene resulting in a constant production of…

A: Genes are the functional segments of DNA. The cluster of these functional segments called genes is…

Q: a mutation that leads to a nonfunctional adenylate cyclase enzyme would lead to what expression…

A: A mutation is a change in the genetic material (the genome) of a cell of a live creature or a virus…

Q: How does the binding of the trp corepressor and the lac inducer to their respective repressor…

A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Arginine is one of the amino acids that serves as a building block for protein synthesis. Arginine…

A: Arginine is one of the amino acids that serves as a building block for protein synthesis. Arginine…

Q: In terms of the efficient use of bacterial resources, explain why it is necessary for lac operon…

A: Lactose can be broken down by E. coli bacteria to produce glucose and galactose. The lac operon…

Q: If an abnormal repressor protein could still bind allolactose, but thebinding of allolactose did not…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: What is the effect of supper repressor (lacI^s) mutations?

A: Lac operon is a transcriptionally regulated system in E.coli which enables the bacteria to utilize…

Q: What is the critical step for controlling gene expression at the posttranslational level. ?

A: The controlling of the gene expression after the transcription or during translation or after…

Q: If there was a mutation such that the lac I protein could no longer bind lactose, but could still…

A: Lac operon is responsible for the metabolism of lactose in bacteria.

Q: For this lac mutant in an E. coli strain, indicate whether B-galactosidase and permease would be…

A: Bacterial genes performing similar functions are typically organized along during a linear serial…

Q: How could the expression of Lactase RNA be turned on and off by transcription factors, activators,…

A: Ans-  Transcription factors are proteins that bind to neighboring DNA and help switch particular…

Q: Where metal-inducible transcription factor 1 (MTF-1) is found ?

A: Metal regulatory transcription factor-1 or metal inducible transcription factor-1 plays a role in…

Q: he lac repressor and the pur repressor are homologous proteins with very similar three-dimensional…

A: Purine repressor (PurR) is a DNA-binding protein that is involved in the transcription process. Its…

Q: What is the function of cAMP in the regulation of the lac operon and how is the production of cAMP…

A: The Lac operon is a set of genes in prokaryotic cells that regulate lactose metabolism. An operator…

Q: How could the increase in a protein that inhibits the activity of sigma-32 lead to an increase in…

A: The "molecular chaperone", dnaK, is induced upon heat shock and promotes ATP-dependent refolding or…

Q: Why it is essential to the positive stimulation of transcription of the lac operon ?

A: Lac operon includes the genes that code for the enzymes involved in lactose catabolism.

Q: Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor.…

A: The lac operon is an operon, or group of genes with a single promoter.The genes in the operon encode…

Q: The lac operon consists of regulatory regions such as the promoter as well as the structural genes…

A: Lac operon is an inducible type of operon system takes place in prokaryotes like E.coli in which…

Q: 15c) Attenuation is a highly efficient mechanism for regulating expression of genes involved in…

A: Attenuation is a regulatory mechanism that is used in bacterial operons to ensure proper…

Q: Suppose the tRNA synthetase responsible for attaching tryptophan to tRNA is muted in a bacterial…

A: In order to maintain correct transcription and translation, bacterial operons use attenuation as a…

Q: The post transcriptional modifications of the mature prokaryotic messenger RNA are essential in…

A: Transcription is a heterocatalytic action of DNA by means of which RNA is synthesized from specific…

Q: Describe the regulation of expression of the lac Z, Y and A genes in E.coli when there is LOW…

A:

Q: Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence…

A: The major carbohydrate source for bacterium are Glucose and Lactose. Glucose is is first choice of…

Q: In addition to the effect on ribonucleotide reductase, hydroxyurea causes changes at the gene…

A: In each living cell, ribonucleotide enzyme (RNR) may be a crucial catalyst that facilitates the…

Q: The lac genotypes are as shown below: P+OcZ-Y+A+// P¬O+Z+Y+A+ (i) The lac operon consists of three…

A: Lac operon refers to the set of genes responsible for uptake and metabolism of lactose. Three…

Q: You know that the mRNA and protein produced by aparticular gene are present in brain, liver, and…

A: The mRNA (messenger Ribonucleic acid) and protein are present in all the three cell types; that is…

Q: In the topic about prokaryotic gene expression regulation, we learned about the LacI-, LacIS, and…

A: The mutation is considered as a way in which there is the occurrence of diversity in genes. The…

Q: The yeast gene SER3, whose product has a role in serine biosynthesis, is repressed during growth in…

A: The yeast cell consists of a cell wall and a plasma membrane. The cell wall is composed of mostly…

Q: A mutant strain of E. coli produces β-galactosidase in both the presence and the absence of lactose.…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes…

A: Lac operon of the bacteria includes the structural genes that code for the enzymes involved in the…

Q: Suppose that  a  new  mutation  lacIes, ('es'  stands  for  ‘extra-strength’)  has been discovered…

A: Operon is the prokaryotic gene regulatory system that involves production of polycistronic mRNA.…

Q: Based on the partial diploid sketched shown in Figure 3, where cross-hatched rectangles and the red…

A: An operon is defined as the working unit of DNA that are carrying a cluster of genes below the…

Q: Repressors are inactivated either by interaction with a small-molecule inducer or by proteolytic…

A: Lac Operon operates in bacteria. It allows the expression of proteins responsible for the catabolism…

Q: Which reaction normally happens in the regulation of the trp operon when high levels of tryptophan…

A: The trp repressor controls the trp operon. When coupled to tryptophan, the trp repressor prevents…

Q: Hemophilia in the royal family was caused by the presence of a mutation in the gene for factor IX…

A: Inheritance or heredity is passing-on one trait form the parents to the progeny by either asexual or…

Q: . a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a…

A: Trp operon is also called as tryptophan operon, a group of genes that encodes biosynthetic enzymes…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.