If the extinction coefficient can be calculated from a single absorbance measurement at a known concentration of a compound, a) why is a calibration curve necessary, even if the measured absorbance is below 1? b) Why should absorbance be less than 2? Please explain in terms of light transmittance.

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Q: Absorbance

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.