If the extinction coefficient can be calculated from a single absorbance measurement at a known concentration of a compound, a) why is a calibration curve necessary, even if the measured absorbance is below 1? b) Why should absorbance be less than 2? Please explain in terms of light transmittance.

If the extinction coefficient can be calculated from a single absorbance measurement at a known concentration of a compound, a) why is a calibration curve necessary, even if the measured absorbance is below 1? b) Why should absorbance be less than 2? Please explain in terms of light transmittance.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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If the extinction coefficient can be calculated from a single absorbance measurement at
a known concentration of a compound, a) why is a calibration curve necessary, even if
the measured absorbance is below 1? b) Why should absorbance be less than
2? Please explain in terms of light transmittance.

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