Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Ⓒ Macmillan Learning
A solution contains DNA polymerase and the Mg2+ salts of dATP, dGTP, dCTP, and TTP.
When added to aliquots of the solution, which of the following DNA molecules would lead to DNA synthesis?
a single-stranded closed circle containing 1000 nucleotide units
a double-stranded linear molecule of 1000 nucleotide pairs with a free 3'-OH group at each end
a double-stranded closed circle containing 1000 nucleotide pairs
a single-stranded closed circle of 1000 nucleotides base-paired to a linear strand of 500 nucleotides with a free
3'-OH terminus
Identify the reasons why the DNA molecule you selected would lead to DNA synthesis.
It is double-stranded DNA.
It has a primer with a free 3'-OH group.
It has an available template strand.
ATP supplies the energy required.
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Transcribed Image Text:Ⓒ Macmillan Learning A solution contains DNA polymerase and the Mg2+ salts of dATP, dGTP, dCTP, and TTP. When added to aliquots of the solution, which of the following DNA molecules would lead to DNA synthesis? a single-stranded closed circle containing 1000 nucleotide units a double-stranded linear molecule of 1000 nucleotide pairs with a free 3'-OH group at each end a double-stranded closed circle containing 1000 nucleotide pairs a single-stranded closed circle of 1000 nucleotides base-paired to a linear strand of 500 nucleotides with a free 3'-OH terminus Identify the reasons why the DNA molecule you selected would lead to DNA synthesis. It is double-stranded DNA. It has a primer with a free 3'-OH group. It has an available template strand. ATP supplies the energy required.
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