Ideal primers for the PCR reaction should have the following feature: they should have a high A-T content the forward primer should be palindromic to the reverse primer they should anneal rapidly only on one target in the DNA template they should have a high G-C content

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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  • Ideal primers for the PCR reaction should have the following feature:
    1. they should have a high A-T content
    2. the forward primer should be palindromic to the reverse primer
    3. they should anneal rapidly only on one target in the DNA template
    4. they should have a high G-C content
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