Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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How would one calculate the protein concentration for each sample if the original protein concentration is 2mg/mL. ?

 

Sample
1
23
4
5
6
7
U1
U2
U3
C
Volume (UL)
Protein Buffer (water)
0
10
20
30
50
75
100
50
50
50
100
90
80
70
50
25
0
50
50
50
Dye
900
900
900
900
900
900
900
900
900
900
A590nm
F
G
H
A450nm A590nm /A450nm Concentration (µg/mL)
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Transcribed Image Text:Sample 1 23 4 5 6 7 U1 U2 U3 C Volume (UL) Protein Buffer (water) 0 10 20 30 50 75 100 50 50 50 100 90 80 70 50 25 0 50 50 50 Dye 900 900 900 900 900 900 900 900 900 900 A590nm F G H A450nm A590nm /A450nm Concentration (µg/mL)
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