How would one calculate the protein concentration for each sample if the original protein concentration is 2mg/mL

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
icon
Concept explainers
Question

How would one calculate the protein concentration for each sample if the original protein concentration is 2mg/mL. ?

 

Sample
1
23
4
5
6
7
U1
U2
U3
C
Volume (UL)
Protein Buffer (water)
0
10
20
30
50
75
100
50
50
50
100
90
80
70
50
25
0
50
50
50
Dye
900
900
900
900
900
900
900
900
900
900
A590nm
F
G
H
A450nm A590nm /A450nm Concentration (µg/mL)
Transcribed Image Text:Sample 1 23 4 5 6 7 U1 U2 U3 C Volume (UL) Protein Buffer (water) 0 10 20 30 50 75 100 50 50 50 100 90 80 70 50 25 0 50 50 50 Dye 900 900 900 900 900 900 900 900 900 900 A590nm F G H A450nm A590nm /A450nm Concentration (µg/mL)
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 18 images

Blurred answer
Knowledge Booster
Proteins
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY