How does the processing of N-linked oligosaccharides and lysosomal proteins differ from that of secreted and plasma membrane proteins in the Golgi apparatus?

Why it is important for the cell membrane to be amphipathic ?

Explain the presence of the protein identified by peptide keratin, type I cytoskeletal ?

The trans-Golgi network is the site of multiple sorting processes as proteins and lipids exit the Golgi complex. Compare and contrast the sorting of proteins to lysosomes with the packaging of proteins into regulated secretory vesicles such as those containing insulin. Compare and contrast the sorting of proteins to the basolateral versus apical cell surfaces in MDCK cells and in hepatocytes.

Prokaryotes and eukaryotes have different Ribosomes that are involved in this process. Which type of cells express the 50S subunit? How have we used this in medicine?

Why peripheral membrane proteins can be studied in absence of membrane and isolated under milder condition?