How does the action and mutagenic effect of 5-Bromouracil differ from that of nitrous acid? explain.

Q: ADH1A alcohol dehydrogenase 1A (class I) Describe a way in which the gene can be manipulated to…

A: * The gene ADH1A alcohol dehydrogenase  gene encodes a member of the alcohol dehydrogenase family.…

Q: What is Ehler Danlos Syndrome (EDS)? What is the biochemical abnormality? What are the different…

A: Genes are responsible for the expressed characteristic of a cell. A mutation in the gene that is…

Q: why do cells require RNAi？

A: A biological mechanism known as RNA interference (RNAi) involves the involvement of RNA molecules in…

Q: What is the role of his119 in the RNase-catalyzed hydrolysis of RNA, as indicated in the Figure…

A: Enzyme are proteins that catalyse biochemical reactions. The substrate binds to active site of the…

Q: What are the differences between synthesis of phosphatidylethanolamine in prokaryotes and…

A: Glycerol is a polyol compound that is made up of more than one hydroxyl group. Glycerol is a…

Q: 2. Design a primer pair using NCBI Primer-BLAST for gene RNA 2',3'-cyclic phosphate and 5'- OH…

A: Forward primer:A primer that binds to antisense or plus strand of DNA is called as forward…

Q: what is the biochemical basis for affinity purification of a recombinant protein using a 6X his tag…

A: The DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors…

Q: Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA…

A: Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA…

Q: Why is Molisch's test used for the determination of the presence of pentose in the yeast RNA…

A: Carbohydrates  are the polyhydroxy ketones or polyhydroxy aldehydes or their derivatives. In…

Q: Propose a role for the positively charged guanidinium nitrogen atom in the cleavage of…

A: The urea cycle is a series of biochemical reactions which involve the formation of urea (NH2)2C0…

Q: What is the primary and secondary structure of ubiquitin-conjugating enzyme?

A: Ubiquitin is a 76 amino acid polypeptide that is found in a eukaryotic cell. ubiquitin is served…

Q: Discuss the Pharmacokinetics of morphine sulfate using the correct sequence you  have chosen in…

A: Morphine is a drug that targets a specific type of receptor that affects multiple processes. It does…

Q: How many times does the enzyme Mbol cut between positions 1426-2789 inclusively of the sequence with…

A: The Mbol is a restriction enzyme. Restriction enzymes cleave the double-stranded DNA at the…

Q: Why do amino acids other than methionineoccur in the N-terminal position of proteins from…

A: The N-terminal end of a protein is defined as the start point of the protein. It is called the…

Q: Selenocysteine and pyrolysine differ in the way that they areconverted to their cognate charged…

A: There are 20 Amino acids and selenocysteine and pyrrolysine are 21 and 22 amino acids respectively.

Q: 5-Bromouridine is known to induce mutations in DNA. One of the characteristics of this compound is…

A: 5-Bromouridine is a uridine subordinate with a Bromo substituent at the fifth carbon. Br -Urd is…

Q: Zidovudine and Stavudine are nucleoside reverse transcriptase inhibitors which consists of a…

A: Nucleoside reverse transcriptase is  RNA-dependent DNA polymerase enzyme which synthesizes the DNA…

Q: Why are the activated nucleotides in nucleic acid polymerization reactions 5’-triphosphates?

A: According to the question, we have to explain the reason for the activated nucleotides in nucleic…

Q: Is Lobry Van Bruyn Transformation the same as the Tautomerization of Fructose during Fehling’s test?…

A: The tautomerization process is crucial because it converts the ketone group of the ketose into a…

Q: What are revision products? What type of reaction is it? What is the common intermediate reversion…

A: Carbohydrates are complex biomolecules made up of simple building units/monomers called sugars or…

Q: Why is the position of Cys 58  important in 3GRS(GLUTATHIONE REDUCTASE)? When Cys 58  is mutated to…

A: Dear Students, I have downloaded the PDB ID 3GRS structure from https://www.rcsb.org/ This is the…

Q: What is an anomer? Are these two models anomers? Explain.

A: Stereoisomerism or spatial isomerism is a form of isomerism in which molecules have the same…

Q: What is the formula for transformation efficiency?

A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…

Q: 4. 5-methylcytosine results from which of the following eve

A: DNA methylation is a process in which the methyl group (CH3) is added to the nitrogenous base.…

Q: The term ‘salvage pathway’ is a way in which a macromolecule is synthesized from intermediates in…

A: Nitrogenous bases are heterocyclic, planar, and relatively water insoluble aromatic compounds. There…

Q: Mutants of Neurospora crassa that lack carbamoyl phosphate synthetase I (CPS I) require arginine in…

A: Carbamoyl Phosphate : It is an anion of biochemical significance. In land-dwelling animals, it is an…

Q: In biochemical and structural terms, how does the cellular form of the prion protein differ from the…

A: There are different macromolecules present in the living organism. Of which proteins are one of the…

Q: What are the low-molecular-weight precursors needed for the de novo biosynthesis of the purine ring,…

A: Purine nucleotides are synthesized in the body via de novo synthesis or salvage pathway. Adenine and…

Q: 1. Reduction of thioredoxin 2. Reduction of ribonucleotide reductase 3. Oxidation of thioredoxin…

A: Ribonucleotide reductase is an enzyme that reduces ribonucleotides to deoxy-ribonucleotides. It…

Q: Why is there no net gain of methionine if homocysteine isconverted to methionine with…

A: Methionine is an amino acid which is found in meat, fish and dairy products and it plays an…

Q: What cofactors are necessary for methyltransferases t Homocysteine Methionine o work?

A: Methyltransferases are primarily responsible for the transfer of methyl groups to other molecules.

Q: comparing the synthesis of pyrimidines vs. purines

A: The purines and pyrimidines are the two families of nitrogenous bases , which are make up nucleic…

Q: The text states that in E. coli ribonucleotide reductase Tyrosine-122 was identified as the source…

A: Ribonucleotide reductase (RNR) is a key enzyme that mediates the synthesis of deoxyribonucleotides,…

Q: Discuss the main similarities and differences in the nucleic acid sequencing methods known as…

A: DNA sequencing is the process of determining the sequence of nucleic acids, which are the basic…

Q: 5-Azacytidine is a reagent that suppresses DNA methylation through its conversion to…

A: Regulation of gene expression is a biological process where the cells perform specific mechanisms…

Q: Human ribonucleotide reductase has two allosteric sites, the S site and the A site. What is the…

A: Human ribonucleotide reductase in a mammalian enzyme that is responsible for the conversion of RNA…

Nitrogen metabolism

Nitrogen metabolism is the synthesis, utilization or uptake, breakdown, and excretion of nitrogenous compounds in the body. The nitrogen metabolism encompasses nitrogen fixation, biosynthesis, and the breakdown of amino acids, purines, and pyrimidine. Since nitrogen-containing compounds are required for various life processes, nitrogen is an important element of life.