How do you know if the ethyl acetate you get at the end of the experiment is pure? A)We add water, if it mixes, it is pure, B)If we see two spots when we look at TLC, it is naive, C)We look at the boiling point, D)We add desiccant, if it doesn't stick to the bottom of the beaker, it is pure, E)We look at the smell,

Part B: Miscibility of liquids 1. Place 10 drops of propan-2-ol into each of two DRY semi-micro test tubes. 2. Add 10 drops of water to one test tube and 10 drops of cyclohexane to the other test tube, shake and observe whether the two liquids in each test tube are miscible. Record your results then discard the contents of the test tubes into the organic waste container. 3. Place 10 drops of water in a clean semi–micro test tube and add 10 drops of cyclohexane to it. Record whether the two liquids are miscible. If two layers form (i.e. the liquids are immiscible) add a drop of blue food dye to the mixture and shake, then allow the two layers to reform. Based on your observation, decide whether the food dye is more soluble in water or cyclohexane and then discard the mixture into the non–halogenated waste container. Record whether the combinations of liquids shown below are miscible. Water Cyclohexane Propan-2-ol Cyclohexane

1. As shown below, a student obtains two quality chromatograms for her distillate and pot samples, containing methanol and cyclohexanol. Match Chromatograms A and B with distillate or pot, and explain your conclusion. Circle your response: Chromatogram A A: Distillate or Pot Explain your answer: t=0 Chromatogram B B: Distillate or Pot

q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762