How do I tell whether or not a bacteria is gram + or gram - ?

How would you dilute a stock solution of bacteria with an OD600 of 2.5 down to a solution that has an OD600 of 0.25?

What are some of the reasons for a gram-variable reaction?

There are major differences between the bacteria and the archaea but both groups were historically grouped together. i) summarize the similarities between the bacteria and archaea, ii) what are the differences between the two groups that allow us to distinguish them?

14) A streptobacillus shaped bacteria have the following shape/sructure: a) long chain of capsule/rodshaped cells b) long chain of spherical-shaped cells c) a single spherical-shaped cell d) a single capsule/rod-shaped cell e) none of the above.15) If you are a passenger on the “Magic School Bus” and you want to “explore” the cytoplasmic component of a Gram -ve bacteria, you will have to go through: a) two membranes separated by periplasm and thick peptidoglycan region b) two membranes separated by periplasm and thin peptidoglycan region c) A glycocalyx and a membrane separated by a thin peptidoglycan region d) a thin peptidoglycan region & one membrane e) a thick peptidoglycan region & one membrane 16) If you are a passenger on the “Magic School Bus” and you want to “explore” the cytoplasmic component of a Gram +ve bacteria, you will have to go through: a) two membranes separated by periplasm and thick peptidoglycan region b) two membranes separated by periplasm and thin…

You are a doctor at a field hospital deep in the Amazon jungle. A local villager comes to you sick with nausea, diarrhea and vomiting and you suspect they may have food poisoning. Several different types of bacteria can cause food poisoning including Campylobacter jejuni, Staphylococcus aureus, and Salmonella tyhphirium. How will using a microscope help you to identify which of the three bacteria are responsible for making your patient sick so that you can administer the correct antibiotic?

What shape is the gram negative and gram positive?

It is 0.4 um in length, but that is all you know.  What could you do to determine if this microbe was a bacteria or an archaea?

What is meant when it is said that a bacteria is an obligate anaerobe?

What is the process to perform a Gram Stain?  What happens if you make a mistake on a step (Think about different mistakes you could make)? What are the proper results?  What structures do Archaeans use to attach to surfaces (Look like treble hooks)? What are bacterial inclusions? What are the similarities and differences in Gram - cells and Gram + cell structure? What is the function of ribosomes and what are they mad up of?  What component of bacterial cells helps to combat/regulate osmotic forces?  What are Koch’s postulates? What are they used for?  Explain the process of endospore formation in endospore-producing organisms. What are the major components of the bacterial & eukaryotic cell? What is/are a pilus/pili and what do microbes use them for? What is the prokaryotic flagellum made up of?