How could the following experiment be set up?  Students haveing learned the optimum sugar concentration is 20%; students next decide to investigate whether different strains of yeast ferment glucose to produce different amounts of alcohol.

Which of the following test will be useful for initial distinction between Which of the following test will be useful for initial distinction bet Salmonella and Escherichia? Lactose fermentation in MacConkey Motility H2S production in Indole medium Glucose fermentation in KIA

Arrange the following steps in the experiment regarding the effect of temperature on fermentation (anaerobic respiration) 1 4 Sugar and water are mixed Measure the height of foam is measured Test tubes are placed in water bath Yeast and glucose solution are mixed Graph the height vs temperature

2C. A “pulse-chase" experiment using "C-labeled glucose can be carried out on a yeast extract that is maintained under strictly anaerobic conditions. The experiment consists of incubating a small amount of "C-labeled substrate (the pulse) with the yeast extract just long enough for each intermediate in the fermentation pathway to become labeled. The label is then “chased" through the pathway by the addition of excess unlabeled glucose. The chase effectively prevents any further entry of labeled glucose into the pathway. 2 ADP 2 ATP Glucose ---> CH3 2 NAD* 2 NADH 2 Pyruvate H H H-C-OH C=0 CH3 CH3 2 co, 2 Ethanol 2 Acetylaldehyde (1) If [1-"C]glucose (glucose labeled at C-1 with "C) is used as a substrate, will there be any "C in the product (ii) Lactic acid is the final fermentation product in vertebrates, while ethanol is the final fermentation product in yeast. Lactic acid can be converted back into glucose while ethanol can not be. In the space below, use a thermodynamic rationale to…

You design an enzyme assay and choose a substrate concentration equal to the Km of the enzymatic reaction. You measure the rate of the reaction at this substrate concentration to be 75.0 μmol/min. Calculate the Vmax of this reaction in μmol/min. Express your answer with one decimal place.  Your previous attempt suffered from low signal-to-noise ratio when you used a substrate concentration equal to the Km. So you decide to increase the substrate concentration to 0.45 mM. You remeasure the rate of the reaction at this new substrate concentration and find it to be 135.0 μmol/min. Using the same Vmax value found above, calculate the Km value of this enzymatic reaction. Express your answer in μM with one decimal place.  Considering the previous two questions, what would be the rate of the reaction at a substrate concentration of 0.01 mM? Express your answer in μmol/min with one decimal place.