Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Question 1.4
How can you calculate the concentration of a solution with UV-vis
spectroscopy if you do NOT know the molar absorption
coefficient?
(a) Add blue dye to the sample and then subtract the blank
sample's absorbance.
(b) Use Beer's Law by assigning the molar absorption
coefficient a value of 1 (e = 1).
(c) Create a calibration curve which is a plot of absorbance vs
concentration.
(d) Calculate the rate constant.
(e) Divide the measured absorbance by the product of I´e.
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Transcribed Image Text:Question 1.4 How can you calculate the concentration of a solution with UV-vis spectroscopy if you do NOT know the molar absorption coefficient? (a) Add blue dye to the sample and then subtract the blank sample's absorbance. (b) Use Beer's Law by assigning the molar absorption coefficient a value of 1 (e = 1). (c) Create a calibration curve which is a plot of absorbance vs concentration. (d) Calculate the rate constant. (e) Divide the measured absorbance by the product of I´e.
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