Hoechst 33258 is a chemical dye that binds specifically to DNA in live cells, and when excited by UV light, it fluoresces in the visible spectrum. Name and describe one specific method, employing Hoechst 33258, an investigator would use to isolate fibroblasts in the G2 phase of the cell cycle from those fibroblasts in interphase.

Hoechst 33258 is a chemical dye that binds specifically to DNA in live cells, and when excited by UV light, it fluoresces in the visible spectrum. Name and describe one specific method, employing Hoechst 33258, an investigator would use to isolate fibroblasts in the G2 phase of the cell cycle from those fibroblasts in interphase.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter10: Cell Reproduction

Section: Chapter Questions

Problem 39CTQ: Rb is a negative regulator that blocks the cell cycle at the G1 checkpoint until the cell achieves a...

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