HEPES ((4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid) is a commonly used cell culture buffer with a pka of 7.5. If you need to prepare 500 mL of 0.2 M HEPES, pH 6.9, how much would you need of the following stock chemicals: 1.0 M HEPES, pH 8.0; 6 M HCI; 6 M NaOH; and distilled H2O?

HEPES ((4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid) is a commonly used cell culture buffer with a pka of 7.5. If you need to prepare 500 mL of 0.2 M HEPES, pH 6.9, how much would you need of the following stock chemicals: 1.0 M HEPES, pH 8.0; 6 M HCI; 6 M NaOH; and distilled H2O?

Chemistry

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ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

HEPES ((4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid) is a
commonly used cell culture buffer with a pKa of 7.5. If you need to
prepare 500 mL of 0.2 M HEPES, pH 6.9, how much would you need of
the following stock chemicals: 1.0 M HEPES, pH 8.0; 6 M HCI; 6 M
NaOH; and distilled H2O?

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