he steps to replicate the identified spike protein are as follows: . Find the CDNA sequence of the spike and make primers accordingly. Make CDNA from the viral RNA using the primers and then amplify this CDNA. . Clone the amplified DNA into a bacterial expression vector. .Express the spike protein in bacterial expression system. ( If this is not working because bacteria doesn't ave post-translational modification mechanism, then clone the spike gene in a mammalian expression ector) Express and purify spike protein from the above expression vector system. This way you can identify and replicate the identified protein spike.
he steps to replicate the identified spike protein are as follows: . Find the CDNA sequence of the spike and make primers accordingly. Make CDNA from the viral RNA using the primers and then amplify this CDNA. . Clone the amplified DNA into a bacterial expression vector. .Express the spike protein in bacterial expression system. ( If this is not working because bacteria doesn't ave post-translational modification mechanism, then clone the spike gene in a mammalian expression ector) Express and purify spike protein from the above expression vector system. This way you can identify and replicate the identified protein spike.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:The steps to replicate the identified spike protein are as follows:
1. Find the CDNA sequence of the spike and make primers accordingly.
2. Make CDNA from the viral RNA using the primers and then amplify this CDNA.
3. Clone the amplified DNA into a bacterial expression vector.
4. Express the spike protein in bacterial expression system. ( If this is not working because bacteria doesn't
have post-translational modification mechanism, then clone the spike gene in a mammalian expression
vector)
5. Express and purify spike protein from the above expression vector system.
This way you can identify and replicate the identified protein spike.
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