hat is an ONPG test? WHat is he principle behind this assay.

Q: Why are the correct primer sequences essential for successful amplification?

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: In 1-2 sentences each, state how the UvrA and rfa phenotypes make Ames test strains more sensitive…

A: Ames test This test uses various strains of bacteria that may carry a mutation. One of the commonly…

Q: Explain the methodology, rationale, and challenges in the control of Triatomine vectors.

A: Triatomine: Triatominae bugs are the vectors of Chagas disease. These belong to the Reduviidae…

Q: What are the different categories of cell viability assays? Describe one of them briefly. Define the…

A: Cell viability assays are the assays which are mainly used to determine or find out the number of…

Q: Why is the restriction enzyme digestion step critical in 3C assays?

A: Genetic information is stored in DNA and we often consider the DNA molecule only as a linear…

Q: How can the binding assay approach be utilized to match coding triplets with their amino acid…

A: Live cells use the genetic code to turn information encoded in genetic material (DNA or mRNA codon…

Q: For the beta-galactosidase assay, as controls, you plate bacteria known to be able to digest lactose…

A: the above experiment is about the molecular screening process to check the gene transformation

Q: what is the biochemical basis for affinity purification of a recombinant protein using a 6X his tag…

A: The DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors…

Q: Why is Molisch's test used for the determination of the presence of pentose in the yeast RNA…

A: Carbohydrates  are the polyhydroxy ketones or polyhydroxy aldehydes or their derivatives. In…

Q: What is the possible cause of the presence of an extra band in the results of DNA extraction and PCR…

A: This finding suggests that formation of multiple bands in non-denaturing gel electrophoresis is a…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: V-max is correctly defined as: a) Km b) The fastest velocity achieved during an enzyme assay,…

A: The enzyme is a class of protein except for ribozymes (class of RNA) increase the rate of the…

Q: hat is the similarity of experimen vitamin c assay and the clearance of a molecule like glucose by…

A: A vitamin assay is the measurement of the activity or presence of a specific component in a specific…

Q: What is Folate Trap ? Explain the importance of Folate Trap ?

A: In a human body due to deficiency of vitamin B12 folic acid is rendered inactive this phenomenon is…

Q: What is the biochemical basis of optochin susceptibility testing?

A: There are various biochemical tests which are used for differentiating one type of bacteria from the…

Q: Why is the DNS method used in glucose assay?

A: 3,5-dinitrosalicylic acid (DNS) measure has been broadly utilized for the estimation of diminishing…

Q: Why can we use temperature sensitive genetic screening to identify particular mutants within a…

A: Temperature-sensitive (TS) mutations are rare alleles that are used to study the expression of…

Q: Can you please explain what is happening in this figure? Please address controls, experimental…

A: An article titled "Distinct sets of enhancers activate transcription from the hkCP and dCP in S2…

Q: How can the foreign protein be modified to facilitate its purification?

A: Protein purification is a progression of cycles proposed to confine one or a couple of proteins from…

Q: What is the difference between in-ovo and ex-ovo types of CAM Assay?

A: The CAM test is a robust approach that can be used to analyze the involvement of new chemicals and…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: What is the aim of hydrolyzing the RNA before doing biochemical tests?

A: RNA hydrolysis: It is a process of breakdown/digestion of RNA molecules by breaking phosphodiester…

Q: The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific…

A: Lambda phage exhibits a lytic or lysogenic cycle depending on the expression of certain genes.

Q: How are molecular probes prepared?

A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…

Q: sts?

A: Nucleic acids  are of two types: RNA and DNA  RNA  It is referred to as ribonucleic acid. It is…

Q: How is telomerases related to ageing? How Can telomerase- related ageing be addressed…

A: Humans lose physiological and psychological function as they age, which is a normal process. The…

Q: Various antimicrobial drugs to treat microbial infection have diverse mechanism of action. Consider…

A: Since we only answer 1 question in case of multiple question, we’ll answer the first question as the…

Q: Explain the Triplet Binding Assay ?

A: Introduction  In 1964, two eminent scientists Marshall W. Nirenberg and Philip Leder carried out the…

Q: Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid…

A: Bradford assay: It is a widely used method for measuring total protein concentration and different…

Q: Describe the rationale behind the electrophoretic mobility shift assay.

A: Nucleic acid is serving as primary information carrying molecule in cells. There are two types of…

Q: of COVID-19 in samples? Which is best in your

A: Coronavirus (COVID-19) is a kind of disease which is caused by Severe acute respiratory syndrome…

Q: What is in situ hybridization used for and how  does In Situ Hybridization detect?

A: Hybridization, in general, is a process during which a moiety is formed having combined traits of…

Q: How is signal amplification achieved in enzyme-linked immunosorbent assays?

A: Enzyme-linked immunosorbent assays or ELISA refers to the techniques to detect antibodies, hormones,…

Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?

A: Introduction: RNA is a type of nucleic acids that is a genetic material in some viruses.

Q: the benefit of hydrolyzing RNA before doing biochemical tests

A: Biochemical hydrolysis is a chemical reaction in which a water molecule is consumed for the bond…

Q: When Zidovudine (3'-azidothymidine or AZT) is added directly to an assay measuring the rate of DNA…

A: Zidovudine (AZT) is a drug used to treat (mainly prolong the life) AIDS. The full chemical name of…

Q: CCCP is observed to have caused increase the SDH activity in DCIP assay. Explain why

A: Succinate dehydrogenase (or Electron Transport Chain advanced II) has been the topic of a centered…

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