Genetic Mutations and Tests Across 3 The second phase of a PCR reaction where the primers bind to the target DNA sequence. 5 Chromosomal mutation that results from non-homologous chromosomes undergoing recombination 9 A prenatal test performed on certain pregnant women by inserting a needle into the womb and retrieving amniotic fluid. 12 The heat-resistant DNA polymerase III enzyme used in PCR reactions. 13 Mutations that are caused by external sources of DNA damage (like radiation) 14 The "P" of the RFLP test. 15 Mutations that change a single amino acid within the protein eing made. 16 Mutations that damage the DNA in eggs or sperm and can be passed down to future generations.

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5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…

Formation of a recombinant DNA molecule. 1 GAATTC GAATTO CITAAG CTTAAG double-stranded DNA CAATTO BAATTO CTTAA CITAAT (| AATTC GI AATTC CTTAA IG GHATIC СТТАА G In the above diagram, 1' represents the O sticky ends re striction sites primer O restriction fragments