For the method of RNA sequencing (RNA-Seq), which of the followingis the correct order of steps?a. Isolate RNAs, synthesize cDNAs, fragment RNAs, sequencecDNAs, align cDNA sequencesb. Synthesize cDNAs, sequence cDNAs, isolate RNAs, fragmentRNAs, align cDNA sequencesc. Isolate RNAs, fragment RNAs, synthesize cDNAs, sequencecDNAs, align cDNA sequencesd. Synthesize cDNAs, isolate RNAs, fragment RNAs, sequencecDNAs, align cDNA sequences

Q: You have sequenced the genome of a new E. coli strain that you discovered during the metagenomics…

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: In automated sequencing, you are given a printout of the sense strand of your DNA.  the first thing…

A: Reading frame is a method to read the DNA sequence to decode the amino acids sequence.

Q: 1. Look at the cut DNA sequences shown below in i) and ii). a) What restriction enzyme(s) was/were…

A: To help you better understand the answers above, here are non-technical explanations of each…

Q: The in situ hybridization method is based on Select one: a. labeling specific neuronal components…

A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

Q: In order to construct a genome library, which statement most accurately describes the correct order…

A: Genomic DNA is an entity's chromosomal DNA that serves as a representation of the entirety of its…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: How is ribotyping similar to rRNA sequence analysis? How do the two techniques differ? Do you think…

A: RFLP is a method to check genetic markers in the genome of organisms. The genetic markers are…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Introduction The Process Of Transcribing A Piece Of DNA Into RNA Is Known As Transcription.…

Q: The schematic diagram below shows the functional organization of transcribing RNA polymerase. Match…

A: Transcription is the next step after replication in the central dogma of biology. It is the process…

Q: You construct a translational reporter gene fusion of your favourite gene. How can you assess…

A: Translational Gene Fusion is employed to live protein localization by quantifying promoter activity.…

Q: 1) A molecular biologist creates a form of RNA polymerase that has the same proofreading ability as…

A: A polymerase is an enzyme that helps to synthesizes long chains of polymers or nucleic acids. It…

Q: With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy…

A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…

Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

A: Introduction :- Restriction enzymes, also known as restriction endonucleases, are enzymes that can…

Q: Sanger sequencing (aka the chain termination) a. is a method for for determining the sequence of…

A: Without a doubt!Identifying the order of nucleotides in a section of DNA may be accomplished by the…

Q: We have mRNA prepared from human cells but PCR needs DNA. What should we do?

A: A polymerase chain reaction (PCR) is a technique that is used in molecular biology to amplify a…

Q: 1)explain why it is far less important for RNA Polymerase to have proofreading activity than it is…

A: Fidelity of replication is the most important for the very existence of an organism. Besides its…

Q: CDNA libraries Select an answer and submit. For keyboard navigation, use the select an answer. a a)…

A: Gene is the primary fundamental unit. These are made up of Deoxyribonucleic acid (DNA). The DNAs…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: The work ‘Hybridization’ in DNA finger printing meansa) Pairing between the nucleotides of DNA…

A: DNA fingerprint is by using the DNA sequence identifies individual a person is called DNA finger…

Q: If the eukaryotic RNAi system involves RNA-RNA interactions, how would one characterize the…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a molecular system found in…

Q: CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene…

A: Design a set of targetting sequences using algorithms. For successful genome editing, the major…

Q: The major products of proofreading by RNA polymerase are dinucleotides rather than mononucleotides.…

A: Introduction: DNA polymerase holds the ability to proofread the strand synthesized during…

Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

A: The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Q: There may be more than one correct answer, select all that apply.  To clone genes, a plasmid vector…

A: Introduction  A plasmid vector is a small, circular piece of DNA that is used as a tool for…

Q: What is a genomic DNA library? A. All DNA fragments identified with a probe O B. A general…

A: Genomic libraries are mostly used for the  sequencing applications. A genomic library contains all…

Q: functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA)…

A: (B)The method which is based on sandwich hybridization of a capture and a detector probes with…

Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can

A: CRISPER system can use Cas protein to manipulate the genome present in human cell.

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Transcription  It is a biological processe where RNA is made by using DNA as a template.

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes…

A: A primer is sometimes known to state because the short sequence of nucleic acids that's the…

Q: 4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for…

A: 1.Three things that a potential vector must have in order to be useful is viral replication origin,…

Q: You are on a research project to study rice. Your want to use reverse transcription PCR to amplify…

A: PCR stands for Polymerase Chain Reaction. It is a laboratory technique used to amplify or create…

Q: "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?

A: Introduction The cDNA library is a collection of mRNA segments cloned into independent vector…

Q: Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: Explain the origin of the signal that is used in the nucleic acid sequencing method known as Ion…

A: As it is known that a growing DNA strand contains a 3'OH end to which a base attaches that usually…

Q: describe one method for sequencing DNA and a method for determining RNA sequencing

A: It means to determine the primary structure of an unbranched biopolymer.

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.