For the method of RNA sequencing (RNA-Seq), which of the followingis the correct order of steps?a. Isolate RNAs, synthesize cDNAs, fragment RNAs, sequencecDNAs, align cDNA sequencesb. Synthesize cDNAs, sequence cDNAs, isolate RNAs, fragmentRNAs, align cDNA sequencesc. Isolate RNAs, fragment RNAs, synthesize cDNAs, sequencecDNAs, align cDNA sequencesd. Synthesize cDNAs, isolate RNAs, fragment RNAs, sequencecDNAs, align cDNA sequences

Q: You have sequenced the genome of a new E. coli strain that you discovered during the metagenomics…

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: In automated sequencing, you are given a printout of the sense strand of your DNA.  the first thing…

A: Reading frame is a method to read the DNA sequence to decode the amino acids sequence.

Q: The in situ hybridization method is based on Select one: a. labeling specific neuronal components…

A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

Q: ou are amplifying both gst::egfp mRNA and 23S rRNA in the PCR steps. These are two different types…

A: The DNA in the nucleus makes different types of mRNA via transcription. Different types of mRNA have…

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A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Introduction The Process Of Transcribing A Piece Of DNA Into RNA Is Known As Transcription.…

Q: automated sequencing is used are given a printout of the sense strand of your DNA.  The printout is…

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Q: Outline the procedure of chromatin immunoprecipitation sequencing, and explain how it was used to…

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Q: What is the methodology in RNA Biology | Taylor & Francis Online

A: The question asks about the methodology used in RNA Biology. It wants to know the techniques and…

Q: 1)explain why it is far less important for RNA Polymerase to have proofreading activity than it is…

A: Fidelity of replication is the most important for the very existence of an organism. Besides its…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

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Q: High throughput whole genome sequencing, RNA-seq and ChIP-seq have all been made possible by the…

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Q: Describe a high-throughput, automated method bywhich millions of DNA templates may be…

A: The process of determining the exact order in which the sequence of the nucleotides are present in…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: Describe in detail the process of RNAI including the major protein involved. What is the role of…

A: Biotechnology is the field that makes use of biology to solve problems undergoing the making of some…

Q: Explain the sequencing-by synthesis (SBS) approach ?

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Q: Identify the mRNA sequence that encodes the protein Design primers that will allow them to amplify…

A: Deoxyribonucleic acid(DNA) is a molecule comprised of two polynucleotide chains coiled around each…

Q: Convert to cDNA and add linkers

A: This is the High-throughput sequencing that analyzes DNA/RNA genome and becomes the standard tool…

Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?

A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…

Q: Explain the steps in RNA Interference. Take note, important enzymes and chemicals are needed in your…

A: RNA interference is a conserved wat=y of gene silencing in many organisms and is also a…

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Q: Genetic engineering and gene therapy are similar fields within genomics. What do they have in common…

A: Genetic engineering and gene therapy are similar fields with genomics. They are both similar as they…

Q: oth microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA…

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Q: What is a genomic DNA library? A. All DNA fragments identified with a probe O B. A general…

A: Genomic libraries are mostly used for the  sequencing applications. A genomic library contains all…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Transcription  It is a biological processe where RNA is made by using DNA as a template.

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes…

A: A primer is sometimes known to state because the short sequence of nucleic acids that's the…

Q: Please answer ASAP RNA sequencing (RNA-Seq) of one sample in one run of a massively parallel…

A: Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers…

Q: "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?

A: Introduction The cDNA library is a collection of mRNA segments cloned into independent vector…

Q: What is an advantage of RNA Seq over microarrays?

A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…

Q: Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: indicate which of the following statements is/are FALSE for qPCR    A. The sequence order of steps…

A: qPCR Quantitative Polymerase Chain Reaction (qPCR) also known as Real-Time Polymerase Chain Reaction…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.