For EMB (Eosin Methylene Blue) agar, what result ‘confirms’ coliforms (positive test)? - The MPN mostly looks to grow E. coli as the indicator species of fecal contamination. Most E. coli is not pathogenic, and you can get many more harmful things like viruses from fecal contamination. Why is E. coli the only thing we look for in the lab to determine whether water is contaminated with fecal matter? (like why not look for the viruses?
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Q3) For EMB (Eosin Methylene Blue) agar, what result ‘confirms’ coliforms (positive test)?
- The MPN mostly looks to grow E. coli as the indicator species of fecal contamination. Most E. coli is not pathogenic, and you can get many more harmful things like viruses from fecal contamination. Why is E. coli the only thing we look for in the lab to determine whether water is contaminated with fecal matter? (like why not look for the viruses?)
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- Q1) What does MPN stand for? Why do you think dilutions are needed to use in the MPN test? -How do you know if a tube is positive or negative? Is it guaranteed that they have coliforms in the positive tubes?I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophileTopic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:
- water sample could be (-) for coliforms when tested by MPN method, could this water still be a major public health threat and why?diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14water sample could be (-) for coliforms when tested by PMF method, could this water still be a major public health threat and why?
- Match the physical and chemical methods of control with its use, mode of action etc. Purify H₂0 Sterilize catheters, Petri plates, syringes, sutures placed in plastic wraps Sporocidal antiseptic Their mode of action is lowering the surface tension of water Antiseptic that kills anaerobic bacteria It is use to preserve cadavers, tissues and organs Isopropilic is the most germicidal Standard of disinfectants Choose... Choose... Choose... Choose... Choose... Choose... Choose... Choose... ◆ ◆ → →A Photo credit: BSP3D- Group 5 Pharm. Bio. Sci 3 Laboratory (S.Y. 2016-2017) Sample is Bacillus subtilis; A-Ciprofloxacin; B-Ampicillin; C-Tetracycline; D- Vancomycin Refer to the image above and answer the following questions: 1. By just looking at the example above, which do you think is the most effective antibiotic for Bacillus subtilis? which is the least effective? and why? 2. How will you measure the zone of inhibition of vancomycin (letter D)? 3. What is the importance of evenly distributing the microbe onto the plate? 3. Subtilisa.explanation on how a cultured bacteria should be able to cause disease in healthy organism once inoculated. b.explanation on how the bacteria should be able to reisolated from the experimentally infected host organism and should show similar properties of the original bacteria
- Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar