Eye solution absobs Ight of wavelength 480 nm, and for this absorption, the molar extinction coefficient is nol cm-. A sample of the dye of unknown concentration is placed in a cuvette of path length 1 cm and the sorbance reading is 0.23. Calculate the concentration of the solution. D0 1.3 x 10 M Db.1.3 x 10 mM DC1.3 pM Dd7.6 x 10 M e 1.3 x 10 uM

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.