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Express the following absorbances in terms of percent transmittances.
(a) 0.0356
(b) 0.379
(c) 0.485
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- (d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?(5) A sample of sodium phosphate is dissolved in water to give 100.00 mL of solution (Solution A). Then this solution is diluted 5 times to give the final solution (Solution B). Solution B is Known to contain 3.920 x 1022 number of PO43- ions, which has an absorption peak at 460 nm and absorbance value of 0.355 in a 1.000 cm cuvette. (i) Calculate the molar absorptivity of Solution B. (ii) What mass of sodium phosphate (MM 163.94 g/mol) dissolved in Solution A.2. Express the following absorbances in terms of percent transmittance. a ) 0.918 b) 0.072
- (a) Prove the relationship between the absorbance and concentration in Beer Lambert's law. What is the absorbance (A) of a sample if the transmittance (T) is 0.347?(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: A = ln == ECL In == where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and Lis the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (1) The concentration of the Coumarin dye is 0.004 mol dm, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mor¹? (T) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by. A = ID==ecL In=-= where lo is the incident light intensity, / is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and Lis the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (1) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹?
- Fish-liver oil is a good source of vitamin A, which is mea-sured spectrophotometrically, after correcting for background, ata wavelength of 329 nm.(a) Suggest a reason for using this wavelength.(b) In what region of the spectrum does this wavelength lie?(c) When 0.1232 g of fish-liver oil is dissolved in 500. mL of sol-vent, the absorbance is 0.724 units. When 1.67X10⁻³g of vita-min A is dissolved in 250. mL of solvent, the absorbance is 1.018 units. Calculate the vitamin A concentration in the fish-liver oilAn aqueous solution of a triphosphate derivative of molar mass 502 g mol-1, was prepared by dissolving 17.2 mg in enough water to make 500 cm3 of solution and a sample was transferred to a cell of length 1.00 em. The absorbance was measured as 1.011. Calculate (a) the molar absorption coefficient;(b) the transmittance. expressed as a percentage.for a solution of twice the concentration.34. In each of the following, enough data are given to calculate the indicated parameter(s). (a) Calculate absorbance given that the transmittance is 0.651. (b) Calculateabsorptivitygiventhattheabsor banceis0.234,bis1.00cm,andtheconcentration is 0.0000391 M. (c) Calculate transmittance given that the absorbance is 0.197. (d) Calculate transmittance given that the percent transmittance is 62.8%. (e) Calculate percent transmittance given that the absorptivity is 13562 L mol cm m", b is 1.00 cm, and the concentration is 0.00000355 M.
- (a) Draw a block diagram of an atomic absorption spectrometer (AAS). Briefly describe the function of each components. (b) What is the process of atomization in atomic absorption spectrometer? Describe the steps of the process in the correct sequence. (c) State TWO interferences that are encountered in atomic absorption spectrometer. Suggest ONE solution to overcome the problem. (d.) What is the main difference, in terms of instrumentation, between atomic absorption spectrometer and atomic emission spectroscopy?Calculate the resolving power required to separate the molecular ions of: (A) C3H6S and C5H12D (B) C18H18O and C18H20N Use masses to 4 decimal places. Answer (A) with four significant figures and (B) with five significant figures.1.4 A 6.25 x 10-³ M solution of potassium permanganate (KMnO4) has a transmittance of 47.4% when measured in a 1.5 cm cell at a wavelength of 625 nm. (a) Calculate the absorbance of the KMnO4 solution (b) Calculate the molar absorptivity of KMnO4