Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence in a DNA sample.

PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein technologies. Describe in detail, the components of a PCR reaction and why they are required

Explain the difference between a DNA profile and a microrarray.

The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.

Quantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNA

1) Describe the ingredients of a PCR reaction.  List different temperatures used in each cycle and tell why each is used.

From a single DNA molecule, calculate how many copies would be produced in 12 cycles of pcr

Describe how Sanger sequencing works. Make sure to mention dNTPs, ddNTPS, template strand, primers:

Describe several methods commonly used for the sequencing of DNA