Explain the above results Investigation Result (normal range - female) Serum folate 2.8 (2.4- 17.5) Serum B12 64 (197 - 771) Serum ferritin 127 (10 - 300) Intrinsicfactor antibodies negative Parietal cell antibodies positive
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Explain the above results
| Investigation | Result (normal range - female) |
| Serum folate | 2.8 (2.4- 17.5) |
| Serum B12 | 64 (197 - 771) |
| Serum ferritin | 127 (10 - 300) |
| Intrinsicfactor antibodies | negative |
| Parietal cell antibodies | positive |
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- RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…1. What is an antiserum? 2. What are the potency requirements in an antiserum? 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B
- If detected in antibody screen testing, which of the following antibodies is NOT considered clinically significant in prenatal patients? Please select the single best answer Anti-M Anti-N Anti-Leb Anti-FyaWhat valence does IgM have in the serum? I selected 5, which is incorrect. Please help.List 3 general component in plasma or serum that will increase phagocytosis in the Neutrophil Function Test.
- Fill in the following table. Blood type Antigens on red blood cells Antibodies in plasma Type A+ Type A - Type B + Type B - Type AB + Type AB - Type O + Type O -An MLS student on her clinical rotation is running an immunofixation electrophoresis procedure on a 49 yo. male. She notes something peculiar in the total serum protein lane or in some systems this is called the SPE lane. In this lane, there is no band where albumin should be. All of the other bands are present. She checks the patient’s chemistry profile to find that his serum albumin is in the normal range.1. Is albumin an anion or cation?2. Which direction should albumin move during electrophoresis, toward the anode or cathode?3. What possible reason or reasons would cause the albumin not to show up on the gel?4. Should the test be repeated and if so how should it be done differently?5. Is it possible that the patient’s Iiver does not produce albumin due to chronic liver disease and that is why it did not show up on the SPE?An MLS student on her clinical rotation is running an immunofixation electrophoresis procedure on a 49 yo. male. She notes something peculiar in the total serum protein lane or in some systems this is called the SPE lane. In this lane, there is no band where albumin should be. All of the other bands are present. She checks the patient’s chemistry profile to find that his serum albumin is in the normal range.1. Is albumin an anion or cation?2. Which direction should albumin move during electrophoresis, toward the anode or cathode?3. What possible reason or reasons would cause the albumin not to show up on the gel?4. Should the test be repeated and if so how should it be done differently?5. Is it possible that the patient’s Iiver does not produce albumin due to chronic liver disease and that is why it did not show up on the SPE? P/S: part 1, 2, and 3 was solved
- Rewrite the following sentences after correction: 1. The N1303k mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. 2. If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates blood type B. 3. Indirect ELISA can detect polygenic gene expression.Weigh the following observation: High titers of antibodies for EBVare found in a leukemia patient; a chronically tired, ill businessman; a healthy military recruit; and an AIDS patient. Comment on theprobable significance of antibodies to EBV in human serum.1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B