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Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion

Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion

B. Pancreatin digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water + 1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO3 2. Place equal size of hard-boiled egg white in all tubes. 3. Place all tubes in water bath with a temperature maintained at 40°C_for 1.5 hours. 4. Add 3 drops of toluene in all tubes and incubate till the next lab period. 5. Observe the size of the egg white in each of the tubes. 6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CUSO4 in each tube. Mix and compare the result of the 4 tubes.
Transcribed Image Text:B. Pancreatin digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water + 1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO3 2. Place equal size of hard-boiled egg white in all tubes. 3. Place all tubes in water bath with a temperature maintained at 40°C_for 1.5 hours. 4. Add 3 drops of toluene in all tubes and incubate till the next lab period. 5. Observe the size of the egg white in each of the tubes. 6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CUSO4 in each tube. Mix and compare the result of the 4 tubes.
B. Pancreatin Digestion TUBE SIZE of egg white Results Purple colored Solution 1(Pancreatin) No change in size No change in size Purple colored Solution 2(Water) No change in size Light-Purple colored Solution 3(water, Pancreatin) (Ctrl) - Blue colored solution Size decreased 4(water, Pancreatin, Na2CO3) greatly
Transcribed Image Text:B. Pancreatin Digestion TUBE SIZE of egg white Results Purple colored Solution 1(Pancreatin) No change in size No change in size Purple colored Solution 2(Water) No change in size Light-Purple colored Solution 3(water, Pancreatin) (Ctrl) - Blue colored solution Size decreased 4(water, Pancreatin, Na2CO3) greatly
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