Experimenters can separate F-actin thin myofilaments frommyosin thick myofilaments. First they homogenize musclecells in a blender (to break cell membranes); then they place thehomogenate in a Ca2+-free “relaxing solution” that contains ATP.Explain why ATP must be present and Ca2+ ions must not bepresent in order to isolate thick and thin myofilaments from eachother.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Experimenters can separate F-actin thin myofilaments from
myosin thick myofilaments. First they homogenize muscle
cells in a blender (to break cell membranes); then they place the
homogenate in a Ca2+-free “relaxing solution” that contains ATP.
Explain why ATP must be present and Ca2+ ions must not be
present in order to isolate thick and thin myofilaments from each
other.

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