Experiment 1.2 - Reaction over time 2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the following table, then plot a curve of product formation against reaction time. Absorbance at 415 nm Cuvette 2+ (PNPP + alkaline phosphatase) 04 0.404 0.716 1 t t t t له t t t t t 1 t t t Time (min) 90 80 70 60 50 40 30 20 10 0 0 10 ܢ.15 20 25+ 30€ 35+ 40€ 50+ 60+ 70€ 80+ 90€ 0 10 0.936 1.066+ 1.1374 1.179 1.203 1.215 1.224 1.228 1.230 1.232 1.234 20 30 40 [PNP] (UM) Cuvette 2+ (PNPP + alkaline phosphatase) 0+ 24.871 45+ 59.194 67.581 72.161 74.871 50 76.419 77.194 77.774 78.032 78.161 78.290€ 78.419 PNP 60 70 ✔ A ✔ e e ✔ A 80 90 100
Experiment 1.2 - Reaction over time 2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the following table, then plot a curve of product formation against reaction time. Absorbance at 415 nm Cuvette 2+ (PNPP + alkaline phosphatase) 04 0.404 0.716 1 t t t t له t t t t t 1 t t t Time (min) 90 80 70 60 50 40 30 20 10 0 0 10 ܢ.15 20 25+ 30€ 35+ 40€ 50+ 60+ 70€ 80+ 90€ 0 10 0.936 1.066+ 1.1374 1.179 1.203 1.215 1.224 1.228 1.230 1.232 1.234 20 30 40 [PNP] (UM) Cuvette 2+ (PNPP + alkaline phosphatase) 0+ 24.871 45+ 59.194 67.581 72.161 74.871 50 76.419 77.194 77.774 78.032 78.161 78.290€ 78.419 PNP 60 70 ✔ A ✔ e e ✔ A 80 90 100
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
why is the shape a hyperbolic curve? Please explain the shape
Transcribed Image Text:3. Briefly describe and explain the shape of the curve in Q2. <
![Experiment 1.2 - Reaction over time
2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the
following table, then plot a curve of product formation against reaction time.
Absorbance at 415 nm
Cuvette 2+
(PNPP + alkaline
phosphatase)
04
0.404
0.716
1
1
t t
t
له
t
t t
t t
1
t t
1
Time
(min)
90
80
70
60
50
40
30
20
10
0
0
10
ܢ.15
20
25+
30€
35+
40€
50+
60+
70€
80+
90€
0
10
0.936
1.066+
1.1374
1.179
1.203
1.215
1.224
1.228
1.230
1.232
1.234
20
30
40
[PNP] (UM)
Cuvette 2+
(PNPP + alkaline
phosphatase)
0+
24.871
45+
59.194
67.581
72.161
74.871
50
76.419
77.194
77.774
78.032
78.161
78.290€
78.419
PNP
60
70
✔
A
✔
e
e
✔
A
80
90
100
2](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcf13683b-5d4d-4f2b-a8e6-42acc81e43d2%2Fad1de719-f22a-475b-bf5a-8186f921fa11%2Fnz1m3w_processed.png&w=3840&q=75)
Transcribed Image Text:Experiment 1.2 - Reaction over time
2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the
following table, then plot a curve of product formation against reaction time.
Absorbance at 415 nm
Cuvette 2+
(PNPP + alkaline
phosphatase)
04
0.404
0.716
1
1
t t
t
له
t
t t
t t
1
t t
1
Time
(min)
90
80
70
60
50
40
30
20
10
0
0
10
ܢ.15
20
25+
30€
35+
40€
50+
60+
70€
80+
90€
0
10
0.936
1.066+
1.1374
1.179
1.203
1.215
1.224
1.228
1.230
1.232
1.234
20
30
40
[PNP] (UM)
Cuvette 2+
(PNPP + alkaline
phosphatase)
0+
24.871
45+
59.194
67.581
72.161
74.871
50
76.419
77.194
77.774
78.032
78.161
78.290€
78.419
PNP
60
70
✔
A
✔
e
e
✔
A
80
90
100
2
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