Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Examine the image of a graph of fraction bound versus ligand concentration. The protein in question binds only one ligand per protein. Different points on
the graph are indicated by lettered boxes. Which lettered box is indicating the value used to represent the binding affinity of the ligand for the protein?
(Indicate your response by clicking on the lettered box.)
A
Y
[L]
D
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Transcribed Image Text:Examine the image of a graph of fraction bound versus ligand concentration. The protein in question binds only one ligand per protein. Different points on the graph are indicated by lettered boxes. Which lettered box is indicating the value used to represent the binding affinity of the ligand for the protein? (Indicate your response by clicking on the lettered box.) A Y [L] D
During Anfinsen's experiments with ribonuclease A, after denaturation of the protein with urea and 2-mercaptoethanol, what was the outcome when both
urea and 2-mercaptoethnal were removed from the system?
Re-shuffling of disulfide bonds occurred
Recovery of activity
Recovery of about 1% activity
The enzyme remained inactive
O000
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Transcribed Image Text:During Anfinsen's experiments with ribonuclease A, after denaturation of the protein with urea and 2-mercaptoethanol, what was the outcome when both urea and 2-mercaptoethnal were removed from the system? Re-shuffling of disulfide bonds occurred Recovery of activity Recovery of about 1% activity The enzyme remained inactive O000
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