Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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18
Question Completion Status:
You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the
minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You
identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You
want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme HindIII and clone the digested DNA into your plasmid
(pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing
the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question.
Chromosome 7q31.1
H
FOXP2
H
H XRAY H
---300kb--
H-Hind!!!
1500bp
600bp
900bp
Size Colony Colony Colony Colony Colony Colony
Standard
5
-
-
2kb
———
-
-
Insert Check-Gel of your samples
ori
imp
Which colony(-ies) is(are) likely to have been white?
O a. Colony 1
O b. Colonies 3 & 4
O c. Colonies 1, 2, 3 & 4
O d. Colonies 5 & 6
O e. All colonies were white.
Save All Answea
Click Save and Submit to save and submit. Click Save All Answers to save all answers.
27
Wells
1kb
200bp
PUC-123
H
IV
*****
expand button
Transcribed Image Text:Question Completion Status: You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme HindIII and clone the digested DNA into your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7q31.1 H FOXP2 H H XRAY H ---300kb-- H-Hind!!! 1500bp 600bp 900bp Size Colony Colony Colony Colony Colony Colony Standard 5 - - 2kb ——— - - Insert Check-Gel of your samples ori imp Which colony(-ies) is(are) likely to have been white? O a. Colony 1 O b. Colonies 3 & 4 O c. Colonies 1, 2, 3 & 4 O d. Colonies 5 & 6 O e. All colonies were white. Save All Answea Click Save and Submit to save and submit. Click Save All Answers to save all answers. 27 Wells 1kb 200bp PUC-123 H IV *****
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