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Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method

Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method

Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
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Section E. Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method pls answer tysm!
Then 9. Determine the molar concentration of sucrose that gives 0% change in weight. Obtain the Ys using the previous formula. 10. Then compute the water potential using the fomula: Yw = Ys + Yp Your answer will be in units of J m (energy per unit volume) which are equivalent to pascal (Pa). Convert to MPa by dividing to 10°. What is the sucrose concentration at which zero percent change in weight is observed? What is the water potential Yw of Tradescantia or Allium сера сells? What is the water potential Yw of the Solanum tuberosum potato cells?
Transcribed Image Text:Then 9. Determine the molar concentration of sucrose that gives 0% change in weight. Obtain the Ys using the previous formula. 10. Then compute the water potential using the fomula: Yw = Ys + Yp Your answer will be in units of J m (energy per unit volume) which are equivalent to pascal (Pa). Convert to MPa by dividing to 10°. What is the sucrose concentration at which zero percent change in weight is observed? What is the water potential Yw of Tradescantia or Allium сера сells? What is the water potential Yw of the Solanum tuberosum potato cells?
A similar experiment is as follows: 1. Obtain 11 (50-ml capacity) beakers and mark from 1-11. In each beaker add 20 ml of one concentration of the following sucrose solutions: Distilled water, 0.1., 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M 3. 2. Using a cork borer, cut 55 cylinders from a large potato or any storage tissue and trim each cylinder to 0.5 cm in length. Place the cylinders in a covered beaker as you cut them to prevent them from drying up. Divide the cylinders in sets of 5. Weigh each set (to 0.01 g). Put one set of cylinders in each of the beakers. 4. 5. Record the fresh weights of each set in Table 6. After 90 minutes, remove the cylinders, gently blot dry with paper towels and reweigh. 7. 6. Subtract the initial weights from the final weights. Divide the difference by the initial weight and multiply by 100 to get the % weight change. Complete Table 6. 8. Plot the% change in weight (y, ordinate) vs. sucrose concentration (x, abscissa) on a graph. Draw the best fit straight line through the points. Table 6a. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 | 0.7 0.8 0.9 1.0 (Molarity) 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.0 Initial Weight (g) 3.61 3.38 3.103.02 2.93 2.70 2.50 1.90 1.80 1.63 1.38 Final Weight (g) A Weight (g) % Weight Change Table 6b. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 0.8 0.9 1.0 (Molarity) 5.00 5.00 5.005.00 5.00 5.00 5.00 5.00 5.00 5.00 5.0 Initial Weight (g) 6.41 6.38 5.80 5.054.50 4.00 3.50 2.90 2.20 1.75 1.38 Final Weight (g) A Weight (g) % Weight Change
Transcribed Image Text:A similar experiment is as follows: 1. Obtain 11 (50-ml capacity) beakers and mark from 1-11. In each beaker add 20 ml of one concentration of the following sucrose solutions: Distilled water, 0.1., 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M 3. 2. Using a cork borer, cut 55 cylinders from a large potato or any storage tissue and trim each cylinder to 0.5 cm in length. Place the cylinders in a covered beaker as you cut them to prevent them from drying up. Divide the cylinders in sets of 5. Weigh each set (to 0.01 g). Put one set of cylinders in each of the beakers. 4. 5. Record the fresh weights of each set in Table 6. After 90 minutes, remove the cylinders, gently blot dry with paper towels and reweigh. 7. 6. Subtract the initial weights from the final weights. Divide the difference by the initial weight and multiply by 100 to get the % weight change. Complete Table 6. 8. Plot the% change in weight (y, ordinate) vs. sucrose concentration (x, abscissa) on a graph. Draw the best fit straight line through the points. Table 6a. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 | 0.7 0.8 0.9 1.0 (Molarity) 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.0 Initial Weight (g) 3.61 3.38 3.103.02 2.93 2.70 2.50 1.90 1.80 1.63 1.38 Final Weight (g) A Weight (g) % Weight Change Table 6b. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 0.8 0.9 1.0 (Molarity) 5.00 5.00 5.005.00 5.00 5.00 5.00 5.00 5.00 5.00 5.0 Initial Weight (g) 6.41 6.38 5.80 5.054.50 4.00 3.50 2.90 2.20 1.75 1.38 Final Weight (g) A Weight (g) % Weight Change
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