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Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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
Transcribed Image Text:Fozyme Action: An Investigation of Lactase Activity
137
PART F EFFECT OF pH ON ENZYME ACTIVITY
Activity of Lactaid at Several pls
Observation with Tes Tape"
Glucose present?
Lactose
90 change
More Grean tello
Green Hellow
NO change
Green
NO
pH 7
Yes, 100 mgloL
pH 2
Yes
7100 mglaL
pH 10
NO
Glucose
tes 300 mglaL
RART G. EFFECT OF AN INHIBITOR ON ENZYME ACTIVITY
Inhibitor Effects
Observation with Tes Tape
Glucose present?
Light Green
Teal
Yellow IGreen
Dark Brown
Lactose
Yes
Lactaid"
NO
Ethanol
Yes
Yes
Glucose
1. What is the optimum pH of the lactose-conversion reaction, as shown by your data?
2 Did ethanol act as an efficient inhibitor of the lactose conversion to glucose and galactose?
3. Summarize your findings about the concentration, temperature, and pH sensititivity of lactase.
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