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Biochemistry
ENZYME ACTIVITY

ENZYME ACTIVITY

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Fozyme Action: An Investigation of Lactase Activity 137 PART F EFFECT OF pH ON ENZYME ACTIVITY Activity of Lactaid at Several pls Observation with Tes Tape" Glucose present? Lactose 90 change More Grean tello Green Hellow NO change Green NO pH 7 Yes, 100 mgloL pH 2 Yes 7100 mglaL pH 10 NO Glucose tes 300 mglaL RART G. EFFECT OF AN INHIBITOR ON ENZYME ACTIVITY Inhibitor Effects Observation with Tes Tape Glucose present? Light Green Teal Yellow IGreen Dark Brown Lactose Yes Lactaid" NO Ethanol Yes Yes Glucose 1. What is the optimum pH of the lactose-conversion reaction, as shown by your data? 2 Did ethanol act as an efficient inhibitor of the lactose conversion to glucose and galactose? 3. Summarize your findings about the concentration, temperature, and pH sensititivity of lactase.
Transcribed Image Text:Fozyme Action: An Investigation of Lactase Activity 137 PART F EFFECT OF pH ON ENZYME ACTIVITY Activity of Lactaid at Several pls Observation with Tes Tape" Glucose present? Lactose 90 change More Grean tello Green Hellow NO change Green NO pH 7 Yes, 100 mgloL pH 2 Yes 7100 mglaL pH 10 NO Glucose tes 300 mglaL RART G. EFFECT OF AN INHIBITOR ON ENZYME ACTIVITY Inhibitor Effects Observation with Tes Tape Glucose present? Light Green Teal Yellow IGreen Dark Brown Lactose Yes Lactaid" NO Ethanol Yes Yes Glucose 1. What is the optimum pH of the lactose-conversion reaction, as shown by your data? 2 Did ethanol act as an efficient inhibitor of the lactose conversion to glucose and galactose? 3. Summarize your findings about the concentration, temperature, and pH sensititivity of lactase.
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