enzyme a. b. C. d. e. changes the apparent Km but has no effect on Vmax. alters Vmax but has no effect on Km. is competitive with respect to substrate for binding to the enzyme active site. Answers a and care both correct. Answers b and care both correct.

Strategies to Regular Enzyme Activity: Give an example of an enzyme that uses the strategy shown and describe how the activity of the enzyme is regulated. Regulatory Strategy 1. Covalent Modification 2. Feedback Inhibition 3. Cascade of Reaction Enzyme Describe how the regulatory strategy is employed

A. How does each of the following irreversible inhibitors interact with the Enzymes? IV. 1. Cyanide 2. Heavy-metal ions B. List an antidote for each of these two poisons and describe how each functions.

There is an enzyme inhibition mechanism that is not too detrimental when viewed from the size of Km, namely the inhibition mechanism.... A. non-competitiveB. non-competitive and uncompetitiveC. in-competitive and uncompetitiveD. in-competitiveE. uncompetitive

Which of the following is incorrect about an enzyme-catalyzed reaction? a. Its progress can be monitored as the disappearance of substrate Ob. Its progress can be monitored as the formation of product OC. The reaction rate can be expressed as the change in [enzyme] with time d. None; all the other choices are correct

b. You conduct some additional experiments to elucidate the starlase metabolic pathway. Your results are shown below. Use this information alongside information from the complementation table above to place the intermediates in the correct order on the pathway. (HINT: use the complementation groups from the table above to help you consolidate information on the tables below. Reference practice question 3 from today's lecture for help). Mutant 1 2 3 4 5 6 Precursor --> [Select] None [Select] Addition to minimal medium starlase + + + + + + --> starlase P + + [Select ] T + O + c. Mutant 2 has a loss-of-function mutation for which enzyme in the starlase synthesis pathway? [Select] E1 E2 E3 E4 Precursor → Intermediate 1 → Intermediate 2 → Intermediate 3 → starlase