EcoRI 4359 Clal - BspDI 23 Hindi 29 Aatll - Zral 4284 Bki 4209 Borl 4205 EcoRV 185 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Bmtl - Nhel 229 Bami 375 Sgrl 409 Hincll 3905 Scal 3844 Bsl 3787 Bsl 3759 Banll 471 Banll 485 gl 528 Sphi 562 EcoNI 622 Sall - Accl - Hincll 651 Prul 3733 Pstl 3607- Brbi 3602 Pshl 712 Asel 3537 Eagl 909 bell 949 Nrul 972 Bsal 3433 BrDI 3420 PBR322 4,361 bp BAPI 1045 Ahdi 3361 HapMI - Bful 1063 PMI 1315 Buml 1353 PIMI 1364 Styt 1369 Aval - Bsobl 1425 PuMI 1438 Acul 3000 AlwNI 2884 Bell 2777 k 2682 Msel 1444 Bigl 1447 Pyuti 1480 rop Begl 1650 Bspl 1664 Ba 1668 Drll 2575 Peil - Al 2473 rbl 2404 Earl 2351 Xmal 2029 Prull 2064 spol - Sapl 2350 Ndel 2295 BAPI 2291 BomBI 2122 Dedl 2162 BsZ171- Aoct 2244/ Bsal 2225 TthI - PI 2217 Figure 1 If your gene of interest was inserted at the Sphi restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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EcoRI 4359
Aatll - Zral 4284
Bei 4209
BsrBl 4205
Clal - BspDI 23
Hindill 29
EcoRV 185
Bmtl - Nhel 229
Sspl 4168
Earl 4155
Acul 4048
Xmnl 3961
Hincll 3905
Scal 3844
BamH 375
Sgrl 409
Banll 471
Banll 485
Вы 3787
Bsl 3759
Bgl 528
Sphl 562
EcoNI 622
Sal - Acct - Hincll 651
Pvul 3733
Pstl 3607
Bartl 3602
Pshl 712
Asel 3537
Eagl 909
Bell 949
Bsal 3433
BarDI 3420
Nrul 972
PBR322
4,361 bp
BstAPI 1045
Ahdi 3361
BspMI - Bfual 1063
PAMI 1315
Bsml 1353
PAMI 1364
Acul 3000
Ori
Styl 1369
Aval - BsoBl 1425
PpuMI 1438
Msel 1444
Bigl 1447
Ppu 1480
AlwNI 2884
Bell 2777
kI 2682
rop
Drdi 2575
Bsgl 1650
BspEI 1664
Pcil - Afl 2473
rBl 2404
Earl 2351
Bspol - Sapl 2350
Ndel 2295
BstAPI 2291
Bsaß 1668
Xmat 2029
Pvull 2064
BsmBI 2122
Dndl 2162
BstZ171 - Acct 2244
Bsal 2225
TthI- PI 2217
Figure 1
If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated in
Figure 1, describe the screening process to select the positive recombinants.
Transcribed Image Text:EcoRI 4359 Aatll - Zral 4284 Bei 4209 BsrBl 4205 Clal - BspDI 23 Hindill 29 EcoRV 185 Bmtl - Nhel 229 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Hincll 3905 Scal 3844 BamH 375 Sgrl 409 Banll 471 Banll 485 Вы 3787 Bsl 3759 Bgl 528 Sphl 562 EcoNI 622 Sal - Acct - Hincll 651 Pvul 3733 Pstl 3607 Bartl 3602 Pshl 712 Asel 3537 Eagl 909 Bell 949 Bsal 3433 BarDI 3420 Nrul 972 PBR322 4,361 bp BstAPI 1045 Ahdi 3361 BspMI - Bfual 1063 PAMI 1315 Bsml 1353 PAMI 1364 Acul 3000 Ori Styl 1369 Aval - BsoBl 1425 PpuMI 1438 Msel 1444 Bigl 1447 Ppu 1480 AlwNI 2884 Bell 2777 kI 2682 rop Drdi 2575 Bsgl 1650 BspEI 1664 Pcil - Afl 2473 rBl 2404 Earl 2351 Bspol - Sapl 2350 Ndel 2295 BstAPI 2291 Bsaß 1668 Xmat 2029 Pvull 2064 BsmBI 2122 Dndl 2162 BstZ171 - Acct 2244 Bsal 2225 TthI- PI 2217 Figure 1 If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.
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