Each peak on the tandem mass spectrogram represents a product ion with a +1 charge. Use the spectrogram and the table of amino acid masses to determine the amino acid sequence of the precursor peptide ion. Intensity Arg 100 175.11 200 O 312.17 300 399.20 400 Mass/charge 500 546.27 603.29 600 Ala Asp Gly Leu Phe Thr 700 Answer Bank Arg Gin His Lys Pro Trp Val Asn Glu lle Met Ser Tyr

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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question 28 // both pictures are attached below. 

Each peak on the tandem mass spectrogram represents a product ion with a +1 charge. Use the spectrogram and the table of
amino acid masses to determine the amino acid sequence of the precursor peptide ion.
4
Intensity
I
Arg
100
175.11
200
312.17
300
399.20
400
Mass/charge
O
500
546.27
603.29
600
Ala
Asp
Gly
Leu
Phe
Thr
700
Answer Bank
Arg
Gin
His
Lys
Pro
Trp
Val
Asn
Glu
Ile
Met
Ser
Tyr
Transcribed Image Text:Each peak on the tandem mass spectrogram represents a product ion with a +1 charge. Use the spectrogram and the table of amino acid masses to determine the amino acid sequence of the precursor peptide ion. 4 Intensity I Arg 100 175.11 200 312.17 300 399.20 400 Mass/charge O 500 546.27 603.29 600 Ala Asp Gly Leu Phe Thr 700 Answer Bank Arg Gin His Lys Pro Trp Val Asn Glu Ile Met Ser Tyr
Residue
Gly
Ala
Ser
Pro
Val
Thr
Cys
Ile
Leu
Asn
Mass (Da)
57.02
71.04
87.03
97.05
99.07
101.05
103.00
113.08
113.08
114.04
Residue
Asp
Gln
Lys
Glu
Met
His
Phe
Arg
Tyr
Trp
Mass (Da)
115.03
128.06
128.09
129.04
131.05
137.06
147.07
156.10
163.06
186.08
Transcribed Image Text:Residue Gly Ala Ser Pro Val Thr Cys Ile Leu Asn Mass (Da) 57.02 71.04 87.03 97.05 99.07 101.05 103.00 113.08 113.08 114.04 Residue Asp Gln Lys Glu Met His Phe Arg Tyr Trp Mass (Da) 115.03 128.06 128.09 129.04 131.05 137.06 147.07 156.10 163.06 186.08
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