Do you expect a Pro → Gly mutation in a surface-loop region of a globularprotein to be stabilizing or destabilizing? Assume the mutant folds toa native-like conformation. Explain your answer in terms of the predictedenthalpic and entropic effects of the mutation on the ΔG for protein foldingcompared to ΔG of folding for the wild-type protein.

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A: The proteins are separated on the basis of their molecular weight by the technique known as Sodium…

Q: 1 0.8 0.6 Mutant y 0.4 Wild-Type 0.2 40 60 80 [ligand] 20

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Q: Myoglobin stores O2 in muscle tissue to be used by the mitochondria only when the cell is in oxygen…

A: Haemoglobin is the oxygen carrying and iron containing protein present in the RBCs (Red Blood Cells)…

Q: proteins are deamidated in an aqueous solution to produce ammonium and aspartate, respectively, from…

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Q: protein via transmembrane protein translocation:

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Q: A different Fab fragment binds to lysozyme with a dissociation constant of Kd=10-6 M. A 1 nM (10-9…

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Q: To better understand the effects of palmitoylation on protein X, researchers want to chemically…

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Q: Disulfide bond formation is a regularly observed protien modification that occurs in the lumen of…

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A: Glycoproteins are consisted of protein and glycan moiety which is involved in several biological…

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A: Protease hydrolyses the peptide bond between the amino acid of a polypeptide chain.

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A: Hi! Thanks for your question. As you have posted multiple sub-parts, we are answering only the first…

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Q: Calculate the concentration of the ligand required to fill 25% of the binding site.

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A: Hi. Thank you for the question, As per the honor code, we are allowed to answer three sub-parts at a…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.