Discuss importance of substrate channeling and metabolon formation in the TCA. What is a metabolon? What is meant by substrate channeling? What is the advantage of substrate channeling through a metabolon? How is this topic relevant for the TCA?

What is the general definition of an uncoupler protein? In the context of oxidative phosphorylation, what do uncoupler proteins do, and how do certain cells utilize them for the benefit of an organism?

What is the principle of LAP?   Question 3 options:   A)  phosphatase activity liberates naphthol and immediately coupled with a diazonium salt which forms an insoluble, visible pigment at sites of phosphatase activity   B)  naphthol couples with fast garnet and forms insoluble maroon deposits at sites of enzyme activity   C)  peroxidase present in the primary granules of the granulocytic line transfers hydrogen from various dye substrates to hydrogen peroxidase which yields a colored derivative of the dye which is localized at the site of the enzyme activity   D)  ferric iron deposits in tissue (present mostly as ferric iron within the storage proteinferritin) then react with the soluble ferrocyanide in the stain

Which coenzymes function as C1 carriers in methanogenesis?Which function as electron donors?