Determine the net charge (to the nearest integer) on the followingpeptide at pH 5 AND pH 12. Estimate the isoelectric point (pl) forthis peptide:H2N-Leu-Gly-Lys-Glu-COOHAssume the pK,s for functional groups are:alpha-amino6.alpha-carboxysidechain-amino (Lys)10.5sidechain-carboxy (Glu)4.2O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =6.6O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =3.1O Net charge at plH 5 and 12 is +2 and -1, respectively, pl =7.5O Nct charge at pH 5 and 12 is +1 and -1. respectively,. pl -4.52.

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Answered: Determine the net charge (to the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Given below is the name of a polypeptide Valyllysylalanylhistidylaspartylphenylalanylglycylglutamate The average pKas of different groups/side chains are shown in the table below: Group/amino acid side chain pKa C-terminus 3.5 3.9 E 4.1 H 6.0 8.4 Y 10.5 K 10.5 R 12.5 N-terminus 9.0 What is the total charge of this molecule at pH 5? [ Select ] What is the total charge of this molecule at pH 9.5? [ Select] > >
Please draw a visual representation
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Determine the net charge (to the nearest integer) on the following peptide at pH5 AND pH 12. Estimate the isoelectric point (pl) for this peptide: H2N-Leu-Gly-Lys-Glu-COOH