design an experiment with a living creature to study and prove " Reciprocal inductive" from developmental interactions
Embryonic induction can be defined as the processes in which one group of cells (or inducing tissue) influence and regulate the development of another group of cells (or responding tissues).There are mainly two components involved in the inductive interactions: Inducer and responders.
Let's understand the reciprocal induction by an experiment on a mouse to observe kidney development.
Kidney development in mouse is controlled through a coordinated reciprocal induction between metanephric mesenchymal (MM) and ureteric bud cells.(UB)
So Metanephric mesenchymal (MM) cells and ureteric bud cells progenitor lines are recombined in a three-dimensional Matrigel implants in Severe combined immunodeficient mice or SCID mice.
(Note:Matrigel is a mixture which is composed of various factors such as laminin,nidogen,collagen and heparan sulfate proteoglycans which is secreted from mouse tumor cells .SCID mice have genetic immune deficiency which affect the B and T cells of mice.There is deficiency of mature B and T cells in SCID mice).
Examination of differential potential is performed to observe changes in phenotype, organization and occurrence of special proteins by the use of immunofluorescence and bright field and electron microscopy.
When both type of cells were grown alone,there was no development into specialized structures.
When both cells were combined,there was organization of cells into simple organoid structures of epithelia which was polarised and comprised of lumens .These lumens were surrounded by capillary like structures.
It was indicated that ureteric bud cells were formed from tubuloid structures and mesenchymal metanephric cells acted as the source of capillary like cells.
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