Describe in detail the vapour diffusion method for crystallising a protein

A spheroidal bacterium with a diameter of 1 μm contains two moleculesof a particular protein. What is the molar concentration of the protein?

Mild, non-ionic detergents (like Triton X-100, with polar but uncharged regions that do not denature proteins) would be required for separation of which of the following proteins from cell membranes? monolayer-associated proteins lipid-linked proteins transmembrane proteins integral proteins peripheral proteins

A solution that is described by the term hypertonic means that it is

A solution containing 3.58 x 1023 molecules/m3 of protein in water is separated from pure water by a membrane 3.20 μm thick.  The diffusion coefficient of the protein through the membrane is 7.15 x 10-18 m2/s.  On average, how many molecules cross 0.0240 μm2 of this membrane each second? [Answer as a positive number with 3 sig digits, but do not enter units with your answer]

An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane (but with no cell wall) is immersed in a beaker containing an aqueous solution. The outside environment concentration consists of 0.01 M glucose and the inside of the cell has a concentration of 5.0 M glucose. The plasma membrane is permeable to water and monosaccharides, but impermeable to the disaccharides. Complete the following for the image below: Is the glucose going down or against its concentration gradient? Is the movement of the solute in the cell going out of the cell?       Down...Yes     Down...No     Against...Yes     Against...No