Describe how electron microscopy is different from bright field microscopy.

The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. 0000 1mm 2mm 3mm 4mm 1mm

In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This makes the user more likely to ram the sample into the lens.  Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?

Does the resolution increase or decrease when closing the diaphragm during microscopy.