d) If the [GTP/[GDP] falls to 2.0, calculate G, keeping all other concentrations the same including succinate from above. If the [GTP/[GDP] increases to 100, calculate G, keeping all other concentrations the same. e) What conclusion can you reach about the direction of the reaction under these cellular conditions? Is this reaction reversible or irreversible?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

Please explain parts D and E only. Reposting for continued responses. This is Biochem. Please explain each part thoroughly.

1. The following reactions can be coupled in the citrate cycle.
kJ/mol
kJ/mol
Succinyl-CoA
GDP + Pi.
succinate+CoA
GTP
Gº=-34.0
Gº= 30.5
a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable?
b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant,
K, for the coupled reaction at 37°C.
c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM,
determine the [Succinate] at equilibrium. (Pay attention to concentration units)
d) If the [GTP/[GDP] falls to 2.0, calculate G, keeping all other concentrations the same including succinate from
above. If the [GTP/[GDP] increases to 100, calculate []G, keeping all other concentrations the same.
e) What conclusion can you reach about the direction of the reaction under these cellular conditions? Is this reaction
reversible or irreversible?
Transcribed Image Text:1. The following reactions can be coupled in the citrate cycle. kJ/mol kJ/mol Succinyl-CoA GDP + Pi. succinate+CoA GTP Gº=-34.0 Gº= 30.5 a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable? b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant, K, for the coupled reaction at 37°C. c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM, determine the [Succinate] at equilibrium. (Pay attention to concentration units) d) If the [GTP/[GDP] falls to 2.0, calculate G, keeping all other concentrations the same including succinate from above. If the [GTP/[GDP] increases to 100, calculate []G, keeping all other concentrations the same. e) What conclusion can you reach about the direction of the reaction under these cellular conditions? Is this reaction reversible or irreversible?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON