Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Please explain parts D and E only. Reposting for continued responses. This is Biochem. Please explain each part thoroughly.

1. The following reactions can be coupled in the citrate cycle.
kJ/mol
kJ/mol
Succinyl-CoA
GDP + Pi.
succinate+CoA
GTP
Gº=-34.0
Gº= 30.5
a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable?
b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant,
K, for the coupled reaction at 37°C.
c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM,
determine the [Succinate] at equilibrium. (Pay attention to concentration units)
d) If the [GTP/[GDP] falls to 2.0, calculate G, keeping all other concentrations the same including succinate from
above. If the [GTP/[GDP] increases to 100, calculate []G, keeping all other concentrations the same.
e) What conclusion can you reach about the direction of the reaction under these cellular conditions? Is this reaction
reversible or irreversible?
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Transcribed Image Text:1. The following reactions can be coupled in the citrate cycle. kJ/mol kJ/mol Succinyl-CoA GDP + Pi. succinate+CoA GTP Gº=-34.0 Gº= 30.5 a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable? b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant, K, for the coupled reaction at 37°C. c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM, determine the [Succinate] at equilibrium. (Pay attention to concentration units) d) If the [GTP/[GDP] falls to 2.0, calculate G, keeping all other concentrations the same including succinate from above. If the [GTP/[GDP] increases to 100, calculate []G, keeping all other concentrations the same. e) What conclusion can you reach about the direction of the reaction under these cellular conditions? Is this reaction reversible or irreversible?
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