Consider the following polypeptide sequence: Gly Val Tyr Ile Val Asp His Phe Thr Gly Asn Tyr Ala Leu Met Glu Asp Lys Aps Val Val Ala Tyr Glu His Ala Pro Lys Leu Asp Asp Phe Val Glu Glu Ala Ley Lys Val Glu Ala Gly Glu Val Pro Ala Ala Pro A) List the expected fragments that will result from proteolytic degradation with trypsin. B) List the expected fragments that will result from the reaction with CNBr. C) List the first three amino acids that will be identified by Edman degradation in the fragments generated in B).

nyiaial liio C cysteine stop phenylalanine serine tyrosine A leucine serine stop tryptophan arginine arginine arginine arginine leucine serine stop leucine proline histidine U histidine proline proline proline leucine C A glutamine glutamine asparagine asparagine lysine lysine aspartate aspartate glutamate glutamate leucine leucine isoleucine threonine serine isoleucine threonine serine C A arginine arginine glycine glycine glycine glycine isoleucine threonine A methionine threonine valine alanine valine alanine G A valine alanine valine alanine G What would be the sequence of amino acids based on the codons found on the following MRNA? 5' AUGCCCUAUAGGUGA3' leucine - Histidine - Arginine - Valine - Cysteine-Arginine | O Tyrosine - Glysine - Isoleucine - Serine - Methionine-Stop | O Methionine - Proline - Tyrosine - Arginine-Stop O Methionine - Proline -Proline - Valine-Stop |

Eukaryotic messenger RNA includes a poly A tail. When the poly(A) tail is less than approximately 30 nucleotides in length, explain why the messenger RNA no longer is protected from nuclease digestion.

A researcher has synthesized two peptides and needs to confirm whether the peptides were synthesized with the correct sequence using mass spectrometry. What is the sequence of the b4 and y7 ions for peptide GAMECHANGER?  The peptide SLISRAEFLAGVRVGV is digested with trypsin. Then the products of the digestion are subjected to ESI TOF-MS in positive ion mode with the peptides in a pH 2 solution. What peptide fragments will result and what are their m/z?